多肽酮树皮对lps刺激的264.7细胞一氧化氮产生的抑制作用

P. Deepa, K. Sowndhararajan, Minju Kim, Songmun Kim
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引用次数: 1

摘要

在传统的医学体系中,杜鹃的树皮已被用于治疗各种疾病。本研究的主要目的是通过抑制脂多糖刺激的RAW 264.7细胞中一氧化氮(NO)的产生来确定牛蒡树皮的抗炎作用。为此,对牛蒡树皮不同溶剂提取物的初步植物化学成分和体外抗氧化活性进行了评价,以筛选出最有效的提取物。甲醇提取物总酚(476.2 mg GAE/g)和类黄酮(129.0 mg RE/g)含量最高,具有较强的抗氧化活性,清除DPPH (IC50为19.52 μg/mL)和ABTS (IC50为10.82 μg/mL)。因此,选择甲醇提取物进行细胞系研究。此外,鸢尾草甲醇提取物能有效抑制LPS (13.1 μM, 80 μg/mL)诱导的RAW 264.7细胞NO的生成。综上所述,牛蒡树皮甲醇提取物中总酚类成分含量较高可能是降低细胞NO水平的原因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
INHIBITION OF NITRIC OXIDE PRODUCTION IN LPS-STIMULATED RAW 264.7 CELLS BY DOLICHANDRONE ATROVIRENS BARK
In traditional systems of medicine, the bark of Dolichandroneatrovirens has been used to treat various disorders. The main aim of this study was to determine the anti-inflammatory effect of D. atrovirens bark through the inhibition of nitric oxide (NO) production in lipopolysaccharide-stimulated RAW 264.7 cells.For this purpose, preliminary phytochemical composition and in vitro antioxidant activities of various solvent extracts of D. atrovirens bark were evaluated to select the most effective extract.The methanol extract of D.atrovirens registered the highest amount of total phenolics (476.2 mg GAE/g) and flavonoid (129.0 mg RE/g)contents witha strong antioxidant activity as measured in DPPH (IC50 of 19.52 μg/mL) and ABTS (IC50of 10.82 μg/mL) scavenging activities.Hence, the methanol extract was selected for cell line study. Further, the methanol extract of D. atrovirens effectively inhibited the production of NO in RAW 264.7 cells induced by LPS (13.1 μM at the concentration of 80 μg/mL). It could be concluded that the presence of higher level of total phenolic components in the methanol extract of D. atrovirens bark might be responsible for reducing the NO level in cells.
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