牛布鲁氏菌病DNA扩增检测技术

S. M. Noor
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引用次数: 0

摘要

布鲁氏菌病是造成重大经济损失的牛病之一,被归类为人畜共患疾病。在牲畜中早期发现布鲁氏菌病对于防止疾病向牲畜和人类传播非常重要。布鲁氏菌病控制的成功取决于快速、灵敏和特异的检测方法。本文综述了几种检测牛布鲁氏菌病的方法。目前,印度尼西亚的布鲁氏菌病检测采用血清学和分离方法。后一种方法是布鲁氏菌病诊断的金标准,但其灵敏度较低。因此,由于DNA扩增分子技术在检测血液、牛奶和精液样本中的布鲁氏菌更为敏感、特异和快速,因此已在许多国家开发并应用于家畜和人类。目前已开发出多种用于检测布鲁氏菌病的DNA扩增方法,包括聚合酶链反应(PCR)、指纹图谱和环介导等温扩增(LAMP)。PCR和LAMP检测布鲁氏菌的灵敏度和特异性均高于传统技术。PCR技术在检测布鲁氏菌的血清型和生物多样性水平上具有优势。此外,PCR试剂比LAMP更便宜,更容易获得,LAMP程序更简单,更快。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
DNA Amplification Technique for Detection of Bovine Brucellosis
Brucellosis is one of cattle diseases which causes a very significant economic loss and categorized as zoonotic disease. Early detection of Brucellosis in livestock is very important to prevent the spread of disease to livestock and humans. The success of Brucellosis control depends on rapid, sensitive and specific detection methods. The aim of this paper is to review several methods of Brucellosis detection in cattle. Currently, the detection of Brucellosis in Indonesia is using serological and isolation methods. The latter method is the gold standard of Brucellosis diagnosis, however, its sensitivity is low. Therefore, molecular techniques with DNA amplification have been developed and applied in many countries both in livestock and humans because they are more sensitive, specific and rapid in detecting Brucella sp in blood, milk and semen samples. Various DNA amplification methods for detection of Brucellosis that have been developed including polymerase chain reaction (PCR), finger printing and loop-mediated isothermal amplificatiom (LAMP). Both PCR and LAMP are more sensitive and specific in detecting Brucella sp than conventional techniques. PCR technique has advantages in detecting Brucella sp species to serotype and biovar levels. In addition, PCR reagents are cheaper and easier to obtain than LAMP eventhough, LAMP procedure is simpler and faster.
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