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引用次数: 0
摘要
在这项工作中,我们使用功能化聚苯胺(PAn)纳米线制造了一个生物传感平台,该平台用于制造三种不同的生物传感器。我们在该平台上固定化了单链DNA寡核苷酸(ssdA),并监测了其与互补链(ssdT)在低至10-18 m的浓度下的结合情况。此外,我们在该平台上固定化了葡萄糖氧化酶,该酶可用于在1μM-20mM的宽浓度范围内对水溶液中的葡萄糖进行电流检测。我们成功地将Lamin A (LA)抗体固定在平台上,并且可以很好地证明其对Lamin A蛋白的反应低至10 μm。因此,建立了通用生物传感平台的效率。
Functionalised polyaniline nanowires: A prospective biosensing platform
In this work we have used functionalized polyaniline (PAn) nanowires for fabrication of a biosensing platform which was exploited for fabrication of three different biosensors. In one effort, we have immobilized single-stranded DNA oligonucletide (ssdA) on this platform and have monitored its combination with the complementary strand (ssdT) at a concentration as low as 10-18 M. Moreover we have immobilized glucose oxidase on this platform that could be used for amperometric detection of glucose in aqueous solution over a wide concentration range of 1μM-20mM. We could successfully immobilize Lamin A (LA) antibody on the platform and its response towards the Lamin A protein could be nicely demonstrated down to a concentration as low as 10 μm. Therefore, the efficiency of the general biosensing platform is established.