农业、农场和肥皂工业分离菌株抗生素与重金属的共耐药研究

Rimsha Dilshad, R. Batool
{"title":"农业、农场和肥皂工业分离菌株抗生素与重金属的共耐药研究","authors":"Rimsha Dilshad, R. Batool","doi":"10.54692/lgujls.2022.0604233","DOIUrl":null,"url":null,"abstract":"In this study, a relationship between antibiotic and heavy metal resistance was estimated among culturable bacterial strains of agriculture farm and soap industry soil.A total of 27 bacterial strains were isolated and screened for their antibiotic and heavy metal resistance by supplementing LB agar medium with variable concentrations of respectivestress. On LB-agar medium, agriculture farm soil harboured more cultivable bacterial strains (17 bacterial strains) as compared to the soap industry soil (10 bacterial strains).Minimum inhibitory concentration of antibiotics for bacterial strains ranged from 20μg/ml to 000μg/ml while MIC of heavy metals had a range of 20μg/ml-2000μg/ml for Nickel, Copper, and Mercury whereas the minimum inhibitory concentration of lead and chromium was up to 10,000μg/ml and 250,000μg/ml respectively. A high rate of co-resistance forStreptomycin with Lead and copper and Ampicillin with lead was observed in 90% of industrial soil bacterial strains. In conclusion, multiple antibiotic resistance and antibioticheavymetal co-resistance in bacteria strains could be due to contamination of soil with any sort of heavy metals or the diversity of population inhabiting that particular site. Antibiotic resistance can also be attributed to the horizontal gene transfer in bacteria.","PeriodicalId":148827,"journal":{"name":"Lahore Garrison University Journal of Life Sciences","volume":"19 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Co-resistance of Antibiotics and Heavy metals in Bacterial Strains Isolated from Agriculture Farm and Soap Industry\",\"authors\":\"Rimsha Dilshad, R. Batool\",\"doi\":\"10.54692/lgujls.2022.0604233\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In this study, a relationship between antibiotic and heavy metal resistance was estimated among culturable bacterial strains of agriculture farm and soap industry soil.A total of 27 bacterial strains were isolated and screened for their antibiotic and heavy metal resistance by supplementing LB agar medium with variable concentrations of respectivestress. On LB-agar medium, agriculture farm soil harboured more cultivable bacterial strains (17 bacterial strains) as compared to the soap industry soil (10 bacterial strains).Minimum inhibitory concentration of antibiotics for bacterial strains ranged from 20μg/ml to 000μg/ml while MIC of heavy metals had a range of 20μg/ml-2000μg/ml for Nickel, Copper, and Mercury whereas the minimum inhibitory concentration of lead and chromium was up to 10,000μg/ml and 250,000μg/ml respectively. A high rate of co-resistance forStreptomycin with Lead and copper and Ampicillin with lead was observed in 90% of industrial soil bacterial strains. In conclusion, multiple antibiotic resistance and antibioticheavymetal co-resistance in bacteria strains could be due to contamination of soil with any sort of heavy metals or the diversity of population inhabiting that particular site. Antibiotic resistance can also be attributed to the horizontal gene transfer in bacteria.\",\"PeriodicalId\":148827,\"journal\":{\"name\":\"Lahore Garrison University Journal of Life Sciences\",\"volume\":\"19 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-11-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Lahore Garrison University Journal of Life Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.54692/lgujls.2022.0604233\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Lahore Garrison University Journal of Life Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.54692/lgujls.2022.0604233","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

在本研究中,估计了农业、农场和肥皂工业土壤中可培养菌株的抗生素与重金属抗性之间的关系。通过添加不同浓度的LB琼脂培养基,对27株细菌进行抗生素和重金属抗性筛选。在lb -琼脂培养基上,与肥皂工业土壤(10株)相比,农业土壤含有更多的可培养菌株(17株)。抗生素对细菌的最低抑菌浓度为20 ~ 000μg/ml,重金属对镍、铜和汞的最低抑菌浓度为20 ~ 2000μg/ml,对铅和铬的最低抑菌浓度分别为10000 μg/ml和25万μg/ml。90%的工业土壤细菌对链霉素与铅、铜、氨苄西林与铅的共耐药率较高。总之,细菌菌株的多重抗生素耐药和抗生素-重金属共耐药可能是由于土壤被任何种类的重金属污染或居住在特定地点的人口多样性。抗生素耐药性也可归因于细菌中的水平基因转移。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Co-resistance of Antibiotics and Heavy metals in Bacterial Strains Isolated from Agriculture Farm and Soap Industry
In this study, a relationship between antibiotic and heavy metal resistance was estimated among culturable bacterial strains of agriculture farm and soap industry soil.A total of 27 bacterial strains were isolated and screened for their antibiotic and heavy metal resistance by supplementing LB agar medium with variable concentrations of respectivestress. On LB-agar medium, agriculture farm soil harboured more cultivable bacterial strains (17 bacterial strains) as compared to the soap industry soil (10 bacterial strains).Minimum inhibitory concentration of antibiotics for bacterial strains ranged from 20μg/ml to 000μg/ml while MIC of heavy metals had a range of 20μg/ml-2000μg/ml for Nickel, Copper, and Mercury whereas the minimum inhibitory concentration of lead and chromium was up to 10,000μg/ml and 250,000μg/ml respectively. A high rate of co-resistance forStreptomycin with Lead and copper and Ampicillin with lead was observed in 90% of industrial soil bacterial strains. In conclusion, multiple antibiotic resistance and antibioticheavymetal co-resistance in bacteria strains could be due to contamination of soil with any sort of heavy metals or the diversity of population inhabiting that particular site. Antibiotic resistance can also be attributed to the horizontal gene transfer in bacteria.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信