Estimation of serum creatinine in biochemistry department, Jigme Dorji Wangchuck National Referral Hospital, Thimphu, Bhutan

Recently at JDWNRH, enzymatic method was introduced in the serum/plasma/urine estimation of creatinine which has high sensitivity1.The enzymatic method employs usage of enzymes in the conversion of creatinine in our body to the final product quinone which is a reddish violet colour and the absorbance of this colour is measured. The colour intensity of the reaction is directly proportional to the concentration of creatinine in sample. This method is advantageous as it is more specific and is not affected by the interfering substance such as bilirubin upto 20mg/dl, haemoglobin upto 200 mg/dl2.