Formulation of Natural Coffee Cream in Increasing Antioxidants as an Effort to Prevent Skin Aging

Skin aging is caused by the excessive production of free radicals in the body, thus causing damage to skin tissue; this also results in damage to skin collagen, which further accelerates the aging process of the skin. The purpose of this study was to determine the effect of giving coffee cosmetic creams on reducing superoxide dismutase (SOD) levels in the skin aging process. This study used a true experiment design with a post-test-only group design research design. The population in this study was an old female Wistar (Rattus norvegicus) weighing 200-250 grams and aged 14-16 months. The overall sample count was 24 female wistars divided for each group was 6 in 4 treatment groups. SOD level data were measured using the ELISA (Enzyme Linked Immune-Sorbent Assay) method. The data obtained were analyzed using the Kruskal Wallis Test and the Mann Whitney Test. The results showed that there was a significant increase in SOD levels (p< 0.05), so it can be concluded that giving coffee cosmetic creams increases SOD levels, meaning that there is an increase in SOD or the main antioxidant enzyme that can ward off free radicals.