黄瓜接种剂Аzotobacter chroococum 2.1植物激素的生物合成及毒力研究

S. Kozar, O. M. Bilokonska, N. Kravchenko
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引用次数: 0

摘要

目标。目的:研究一种新的高效偶氮菌属结合菌的性质(植物激素物质的产生和毒力)。方法。通过生物测定法确定植物激素活性。微生物学(获得一种无色固氮菌2.1的悬浮液,通过在消化培养基上播种来测定滴度;应用了对所研究动物内脏的染色印记涂片进行显微镜观察和在消化培养基上培养组织样本)和病理解剖(确定细菌在动物内脏组织中可能的侵袭性和传播)方法。该研究是在一种近交系白小鼠模型上进行的,每只小鼠口服0.5 cm3无菌等渗氯化钠溶液中含有0.5×109至5×109 CFU的活细菌细胞悬液,腹腔注射剂量为1×109至5×109 CFU/0.5 cm3。采用方差分析方法进行统计计算。结果。一株新菌株chroococum 2.1合成生长素样物质比赤霉素样物质和细胞分裂素样物质更密集。1:100的培养液稀释是最有效的。关于细菌可能致病性的研究结果表明,口服活的固氮细菌细胞悬浮液后,小鼠的行为没有发生变化。试验开始后和15天后对实验动物内脏进行的微生物学检测显示,偶氮菌在温血动物体内不具有传染性、不繁殖和不传播。腹腔和口服嗜绿球菌2.1活细胞培养悬液均未引起细菌侵入小白鼠内脏。未检测到逆转录培养。结论。A. chroococcus 2.1产生生长素、赤霉素和细胞分裂素。揭示了菌株a . chroococum 2.1培养液稀释对试验培养物(小麦胚芽、玉米中子叶和黄瓜子叶)影响的抛物线性质,这是植物激素物质产生的结果。新菌株产生的生长素样物质最为强烈。所得数据表明该菌株对温血动物无毒(腹腔LD50 50亿细胞/小鼠,口服LD50 50亿细胞/小鼠)。根据缺乏毒力的数据,在不考虑毒性、毒性、致敏能力和生态失调作用水平的情况下,A. chroococum 2.1可被认为是非致病性的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
BIOSYNTHESIS OF PHYTOHORMONES AND VIRULENCE OF АZOTOBACTER CHROOCOCCUM 2.1 — CUCUMIS SATIVUS L. INOCULANT
Objective. To study the properties (production of phytohormonal substances and virulence) of a new highly effective strain of associative bacteria of the genus Azotobacter. Methods. Phytohormonal activity was established by bioassays. Microbiological (obtaining a suspension of Azotobacter chroococcum 2.1, determining the titre by sowing on a digest medium; microscopy of stained imprint smears of the internal organs of the studied animals and cultivation of tissue samples on a digest medium) and patho-anatomical (determination of possible invasiveness and dissemination of bacteria in the tissue of internal organs of animals) methods were applied. The study was conducted on a model of outbred white mice, after oral dosing of a suspension of live bacterial cells ranged from 0.5×109 to 5×109 CFU in 0.5 cm3 of sterile isotonic sodium chloride solution per mouse and intraperitoneal dosing ranged from 1×109 to 5×109 CFU/0.5 cm3. Statistical calculations were carried out using analysis-of-variance method. Results. A new A. chroococcum 2.1 strain synthesizes auxin-like substances more intensively than gibberellin-like and cytokinin-like substances. A 1:100 dilution of the culture fluid is the most effective. The obtained results of the studies on the possible pathogenicity of bacteria indicate that no deviations in the behaviour of mice were detected after oral dosing of a suspension of living Azotobacter cells. Microbiological tests of the internal organs of the experimental animals after the beginning of the tests and after 15 days revealed that Azotobacter is not infective, does not reproduce and does not disseminate in the body of warm-blooded animals. Intraperitoneal and oral dosing of a live cell culture suspension of A. chroococcum 2.1 did not cause bacterial invasion into the internal organs of white mice. Retrocultures were not detected. Conclusion. A. chroococcum 2.1 produces auxins, gibberellins and cytokinins. The parabolic nature of the influence of dilutions of the culture liquid of the studied strain A. chroococcum 2.1 on the test cultures (wheat coleoptiles, corn mesocotyles and cucumber cotyledons) was revealed, which is a consequence of the production of phytohormonal substances. The new strain produces auxin-like substances most intensely. The obtained data indicate the avirulence of the strain for warm-blooded animals (LD50 intraperitoneal > 5 billion cells/mouse, LD50 oral > 5 billion cells/mouse). According to the data on the lack of virulence, without taking into account the levels of toxigenicity, toxicity, allergenic capacity and dysbiotic action, A. chroococcum 2.1 can be considered non-pathogenic.
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