{"title":"[尿中β - n -乙酰氨基葡萄糖酶和白蛋白的测定:人与大鼠尿液凝胶过滤方法及效果的比较]。","authors":"M Meier, B Wilke, W Junge","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>A new kinetic method for determination of beta-N-acetylglucosaminidase (beta-NAG, EC 3.2.1.30) in human and rat urine with chlorophenol red-NAG as substrate is compared to two other methods with 4-nitrophenyl-NAG and 3-cresol-sulfonphthaleinyl-NAG respectively as substrates. The impact of gel filtration of urinary specimens on the determination of beta-NAG-activity and of low albumin concentrations in urine is described.</p>","PeriodicalId":76822,"journal":{"name":"Wiener klinische Wochenschrift. Supplementum","volume":"189 ","pages":"44-7"},"PeriodicalIF":0.0000,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Determination of beta-N-acetylglucosaminidase and albumin in urine: comparison of methods and effect of gel filtration in human and rat urine].\",\"authors\":\"M Meier, B Wilke, W Junge\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A new kinetic method for determination of beta-N-acetylglucosaminidase (beta-NAG, EC 3.2.1.30) in human and rat urine with chlorophenol red-NAG as substrate is compared to two other methods with 4-nitrophenyl-NAG and 3-cresol-sulfonphthaleinyl-NAG respectively as substrates. The impact of gel filtration of urinary specimens on the determination of beta-NAG-activity and of low albumin concentrations in urine is described.</p>\",\"PeriodicalId\":76822,\"journal\":{\"name\":\"Wiener klinische Wochenschrift. Supplementum\",\"volume\":\"189 \",\"pages\":\"44-7\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1991-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Wiener klinische Wochenschrift. Supplementum\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Wiener klinische Wochenschrift. Supplementum","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[Determination of beta-N-acetylglucosaminidase and albumin in urine: comparison of methods and effect of gel filtration in human and rat urine].
A new kinetic method for determination of beta-N-acetylglucosaminidase (beta-NAG, EC 3.2.1.30) in human and rat urine with chlorophenol red-NAG as substrate is compared to two other methods with 4-nitrophenyl-NAG and 3-cresol-sulfonphthaleinyl-NAG respectively as substrates. The impact of gel filtration of urinary specimens on the determination of beta-NAG-activity and of low albumin concentrations in urine is described.
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