激发子和丹参诱导豌豆过氧化物酶活性及同工酶谱

R. Katoch, A. S. Mann, B. S. Sohal
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引用次数: 3

摘要

摘要/ ABSTRACT摘要:对白粉病敏感的豌豆(Pisum sativum L.)体内过氧化物酶(PO)活性显著升高。5 mM水杨酸(SA)处理后观察‘Arkel’。接种黄芽孢杆菌孢子后,PO活性显著升高。在抗性(JP-179)和敏感品种中,与水处理对照相比,SA (1 mM)和4-氨基丁酸(1 mM和5 mM)处理没有产生显著的PO活性变化。在抗性品种中,5 mM SA处理120 h后,过氧化氢酶活性显著增加。5 mM SA处理和接种黄芽孢杆菌后,两个品种的过氧化物酶同工酶谱都出现了额外的条带。抗白粉病品种中过氧化物酶活性和数量的额外增加可能是病原菌生长的限制因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Enzyme Activity and Isozymic Profile of Peroxidase Induced by Elicitors and Erysiphe polygoni in Pea (Pisum sativum L.)
ABSTRACT A significant increase in peroxidase (PO) activity of powdery mildew (Erysiphe polygoni DC) in the susceptible garden pea (Pisum sativum L.), cv. ‘Arkel’, was observed after treatment with 5 mM salicylic acid (SA). The increase in PO activity after inoculation with spores of E. polygoni was highly significant. Treatment with SA (1 mM) and 4-aminobutyric acid (1 and 5 mM) did not produce significant change in PO activity as compared to water treated control plants in both the resistant (‘JP-179’), and susceptible cultivars. In the resistant cultivar, treatment with 5 mM SA produced significant increase in PO activity after 120 h. The isozymic profile of peroxidase showed additional bands in both cultivars after treatment with 5 mM SA and inoculation with E. polygoni. The additional increase in the activity and number of isoforms of peroxidase in the powdery mildew resistant cultivar may be a limiting factor for the growth of the pathogen.
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