拟南芥对麦氏黑穗病侵染的差异基因研究

Marco M. Plancarte-De la Torre, Kena Casarrubias-Castillo, Celia Robles-Murguía, L. Méndez-Morán
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引用次数: 0

摘要

为了研究拟南芥-麦氏黑穗病整合的致病机制,为了了解植物对麦氏黑穗病致病机制的反应机制,采用减法杂交技术,在接种72 h后构建了差异表达库。对获得的片段进行测序和生物信息学分析,使我们能够定位36个与拟南芥对U. maydis感染有同源性的不同序列,其中一些与光合作用、活性氧、防御和信号等有关,直接或间接参与感染的早期反应。这项工作的结果集中在了解植物与病原体的相互作用,并可以外推到其他重要的农艺模式植物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Differential genes of Arabidopsis thaliana in response to Ustilago maydis infection
Arabidopsis thaliana - Ustilago maydis integrate the pathosystem used to study the plant-pathogen interaction, in order to know the molecular mechanisms involved in the response of the plant to the pathogenesis of U. maydis, a differential expression bank was constructed 72 hours after inoculation, using the subtractive hybridization technique. The fragments obtained were sequenced and subjected to bioinformatic analysis which allowed us to locate 36 different sequences with homology to Arabidopsis thaliana in response to U. maydis infection, several of them with roles in photosynthesis, reactive oxygen species, defense, and signaling among others, involved either directly or indirectly in the early response to infection. The results of this work are focused on understanding the plant-pathogen interaction and can be extrapolated to other model plants of agronomic importance.
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