柔性微流体装置-印章系统与集成的电子传感器实时DNA检测

1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology. Proceedings (Cat. No.00EX451) Pub Date : 2000-10-12 DOI:10.1109/MMB.2000.893735

H. Dobrinski, M. Wilkens, W. Benecke, J. Binder

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引用次数: 5

摘要

描述了用于实时DNA检测的可重复使用的微流体流动池的设计。这种微流体装置-印章系统(MDSS)是由微结构的硅-聚合物-杂化体实现的，该杂化体被压在由薄金属交错电极组成的传感元件上，该传感元件具有固定的捕获DNA，用于杂交检测。杂化温度由采用标准微系统技术制造的集成加热和传感元件控制。利用ANSYS 5.5/FLOTRAN软件进行二维有限元流动分析，优化传感区域内流体流动均匀性和DNA质量输运。

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Flexible microfluidic-device-stamp-system with integrated electrical sensor for real time DNA detection

Describes the design of a reusable microfluidic flow cell for real time DNA detection. This Microfluidic-Device-Stamp-System (MDSS) is realized by a microstructured Silicon-Polymer-Hybrid that is pressed onto a sensing element consists of thin metallic interdigitated electrodes with immobilized capture DNA for hybridization detection. The hybridization temperature is controlled by integrated heating and sensing elements manufactured with standard microsystem technologies. Two-dimensional FEM flow analyses using ANSYS 5.5/FLOTRAN were done to optimize the homogeneities of fluid flow and DNA mass transport in the sensing region.

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1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology. Proceedings (Cat. No.00EX451)

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