哈茨木霉对豇豆品种生长、发病及产量的生物防治作用被炭疽病菌侵染

A. A. I., H. M, SANI, L. A, A. H
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引用次数: 0

摘要

由炭疽杆菌引起的豇豆炭疽病导致尼日利亚豇豆产量减少约75%。哈茨木霉是一种防治真菌病的生物制剂。研究了哈兹黑僵菌对不同品种豇豆生长、发病及产量的影响。选用4个改良豇豆品种IT07K-292-10、it07k - 218 -33、IT98K-205-08、IT99K-573-11和1个地方品种DANMISRA。分别在PDA上培养炭疽菌和哈茨木霉纯分离物。制备4种浓度的哈兹兰菌悬液,分别为1x102分生孢子/ml、1x104分生孢子/ml、1x106分生孢子/ml、1x108分生孢子/ml和对照。播种后22 d,用1 × 107个分生孢子/ml人工侵染豇豆幼苗。试验采用完全随机设计(CRD),每3个重复。结果表明,1 × 108个分生孢子/ml浓度下,侵染品种IT99K-573-11不感病,生物量、种子数显著增加(p<0.05),发病率较低(19.1%);1 × 108个分生孢子/ml浓度下,侵染品种IT99K-573-11生物量、种子数显著增加(p<0.05),炭疽病发病率显著降低(p<0.05);品种IT99K-573-11表现出较强的耐受性,因此推荐农民在林蝇侵染地区种植。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
BIOCONTROL ABILITY OF Trichoderma harzianum ON GROWTH, DISEASE INCIDENCE AND YIELD OF SELECTED COWPEA VARIETIES (Vigna unguiculata L.) INFESTED WITH Colletotrichum lindemuthianum
Cowpea anthracnose caused by Colletotrichum lindemuthianum causes about 75 % yield reduction in Cowpea production in Nigeria. Trichoderma harzianum is a bioagent in controlling fungal diseases. Effect of T. harzianum was investigated on growth, disease incidence and yield of selected varieties of Cowpea infested with C. lindemuthianum. Four improved Cowpea varieties were selected IT07K-292-10, IT07K-318-33, IT98K-205-08, IT99K-573-11 and a local variety DANMISRA. Pure isolate of Colletotrichum lindemuthianum and Trichoderma harzianum were each cultured on PDA. Four concentrations of T. harzianum suspensions were prepared at 1x102 conidia/ml, 1x104 conidia/ml, 1x106 conidia/ml, 1x108 conidia/ml and control . Cowpea seedlings were artificially infested with 1x107conidia/ml of C. lindemuthianum at 22 days after sowing. The experiment was arranged in a complete randomized design (CRD) with three replications. The result indicated that the infested variety IT99K-573-11 treated with T. harzianum at 1x108 conidia/ml was insusceptible and had significantly (p<0.05) increased biomass, number of seeds and low disease incidence (19.1 %) while increase in chlorophyll content, seed number and decreased in the anthracnose incidence was observed at 1x108 conidia/ml concentration. The cultivar IT99K-573-11 appeared tolerant hence is recommendedto farmer for cultivation in C. lindemuthianum infested areas.
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