{"title":"建立反相高效液相色谱法同时测定丙苯酸和头孢地洛辛纯剂型和复方剂型的含量","authors":"Gurumurthy. Telugu, Dr Madhuri. D","doi":"10.46624/ajphr.2019.v7.i9.003","DOIUrl":null,"url":null,"abstract":"A simple and precise, reliable, rapid, reproducible reverse phase HPLC method has been developed and validated for simultaneous estimation of Probenecid & Cefadroxil chromatography was carried out on zorbaxcC18 (4.6*150mm, 5um) column using mixture of ACN: WATER (55:45v/v) as the mobile phase at flow rate of 1.0ml for min the detection wavelength carried out at255nm the retention time of probenecid & cefadroxil was 2.061&2.462respectively. The method produce linear response in the concentration range of 10-50μg/ml of probencid&5-25μg/ml of cefadroxil the method precision for the determination of assay was below 2.0%RSD. The proposed method can be useful in quality control of bulk& pharmaceutical dosage form","PeriodicalId":233230,"journal":{"name":"American Journal of Pharmacy And Health Research","volume":"31 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Development and Validated RP-HPLC Method for Simultaneous Estimation of Probenecid and Cefadroxil in Pure &Combined Dosage Form\",\"authors\":\"Gurumurthy. Telugu, Dr Madhuri. D\",\"doi\":\"10.46624/ajphr.2019.v7.i9.003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A simple and precise, reliable, rapid, reproducible reverse phase HPLC method has been developed and validated for simultaneous estimation of Probenecid & Cefadroxil chromatography was carried out on zorbaxcC18 (4.6*150mm, 5um) column using mixture of ACN: WATER (55:45v/v) as the mobile phase at flow rate of 1.0ml for min the detection wavelength carried out at255nm the retention time of probenecid & cefadroxil was 2.061&2.462respectively. The method produce linear response in the concentration range of 10-50μg/ml of probencid&5-25μg/ml of cefadroxil the method precision for the determination of assay was below 2.0%RSD. The proposed method can be useful in quality control of bulk& pharmaceutical dosage form\",\"PeriodicalId\":233230,\"journal\":{\"name\":\"American Journal of Pharmacy And Health Research\",\"volume\":\"31 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1900-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"American Journal of Pharmacy And Health Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.46624/ajphr.2019.v7.i9.003\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"American Journal of Pharmacy And Health Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.46624/ajphr.2019.v7.i9.003","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
采用zorbaxcC18 (4.6*150mm, 5um)色谱柱，流动相为ACN: WATER (55:45v/v)，流速为1.0ml，时间为min，检测波长为255nm，建立了一种简便、准确、可靠、快速、重现性好的高效液相色谱法，同时测定了苯丙昔和头孢地洛昔的含量。该方法在probencid10 ~ 50μg/ml和头孢地诺酯5 ~ 25μg/ml浓度范围内均呈线性响应，测定精密度小于2.0%RSD。该方法可用于原料药剂型的质量控制
Development and Validated RP-HPLC Method for Simultaneous Estimation of Probenecid and Cefadroxil in Pure &Combined Dosage Form
A simple and precise, reliable, rapid, reproducible reverse phase HPLC method has been developed and validated for simultaneous estimation of Probenecid & Cefadroxil chromatography was carried out on zorbaxcC18 (4.6*150mm, 5um) column using mixture of ACN: WATER (55:45v/v) as the mobile phase at flow rate of 1.0ml for min the detection wavelength carried out at255nm the retention time of probenecid & cefadroxil was 2.061&2.462respectively. The method produce linear response in the concentration range of 10-50μg/ml of probencid&5-25μg/ml of cefadroxil the method precision for the determination of assay was below 2.0%RSD. The proposed method can be useful in quality control of bulk& pharmaceutical dosage form
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