一个实用的单细胞分析方法的机械特性的癌细胞

2017 IEEE 30th International Conference on Micro Electro Mechanical Systems (MEMS) Pub Date : 1900-01-01 DOI:10.1109/MEMSYS.2017.7863481

Tiffany Baëtens, G. Perret, Y. Takayama, M. Kumemura, L. Jalabert, S. Meignan, Chann Lagadec, Fujita Hiroyuki, D. Collard, M. Tarhan

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引用次数: 4

摘要

本研究提出了一种使用硅纳米镊子(SNT)进行细胞力学表征的实用方法。SNT尖端与微流体装置集成，通过侧开口插入通道中。将待表征的细胞注射到微流体通道的入口，并通过连接到出口的真空泵取出。在SNT尖端(传感尖端和压缩尖端)之间捕获后，分析了单元的机械性能，例如直径，刚度和粘性损失。鉴定后，将捕获的细胞释放以捕获另一个细胞。重复捕获和表征步骤使我们能够测试多个细胞，并根据其机械特性区分固定和活的SUM-159-PT癌细胞。

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A practical single cell analysis method for mechanical characterization of cancer cells

This study presents a practical method to perform mechanical characterization of cells using Silicon Nano Tweezers (SNT). Integrated with a microfluidic device, SNT tips were inserted in a channel via a side opening. Cells to be characterized were injected to an inlet of the microfluidic channel and withdrawn by a vacuum pump connected to an outlet. Mechanical properties of a cell, e.g. diameter, stiffness and viscous losses, were analyzed after capturing between the SNT tips (a sensing tip and a compressing tip). After characterization, the captured cell was released to capture another cell. Repeated capturing and characterization steps allow us to test several cells and distinguish fixed and live SUM-159-PT cancer cells based on their mechanical properties.

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来源期刊

2017 IEEE 30th International Conference on Micro Electro Mechanical Systems (MEMS)

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