A novel organophosphorus pesticides sensitive EN-FET

The authors describe a novel organophosphorus pesticide sensitive solid-state sensor, the enzyme modified field-effect transistor (EN-FET), for measurements in liquid media, based on the immobilized enzyme probe (IEP) technique using butyrylcholinesterase. The pH-EISFET with n-channel and silicon dioxide/silicon nitride insulator layers is used as the transducer. Butyrylcholinesterase has been covalently bound onto the gate of pH-ISFET (ion-sensitive FET) using glutaraldehyde and the bovine serum albumin. The organophosphorous pesticide sensitive EN-FET has the advantage of fast response.<>