{"title":"尼日利亚西北部扎里亚市新鲜生肉中单核细胞增生李斯特菌的多重耐药谱及部分毒力基因的分子检测","authors":"S. Olaniyan, J. Kwaga, A. Saidu, U. Usman","doi":"10.21608/aeji.2021.87137.1164","DOIUrl":null,"url":null,"abstract":"Patients and Methods: A crosssectional study was conducted between March and November 2015 to determine the AMR profile and virulence genes associated with Listeria monocytogenes from beef sold in Zaria. A total of 240 raw beef samples were collected from beef retailers in Zaria city, Tudun-Wada, Danmagaji, Sabon Gari, Kwangila, and Samaru. The samples were transported to the Veterinary Public Health laboratory, ABU, Zaria, for processing. The isolates were further identified as Listeria species by biochemical tests. Antimicrobial susceptibility testing was performed by disc diffusion method. Multiplex PCR was used to identify the virulence genes associated with Listeria monocytogenes. The data were analysed using SPSS V23.0 and Microsoft Excel version 2019 for Windows 10. Odd ratio and 95% CI were determined. Value of P< 0.05 was considered significant.","PeriodicalId":261891,"journal":{"name":"Afro-Egyptian Journal of Infectious and Endemic Diseases","volume":"31 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2021-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Multiple Anti-microbial Resistance Profile and Molecular Detection of Some Virulence Genes of Listeria Monocytogenes Isolated from Fresh Raw Meat Retailed in Zaria, Northwestern Nigeria \",\"authors\":\"S. Olaniyan, J. Kwaga, A. Saidu, U. Usman\",\"doi\":\"10.21608/aeji.2021.87137.1164\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Patients and Methods: A crosssectional study was conducted between March and November 2015 to determine the AMR profile and virulence genes associated with Listeria monocytogenes from beef sold in Zaria. A total of 240 raw beef samples were collected from beef retailers in Zaria city, Tudun-Wada, Danmagaji, Sabon Gari, Kwangila, and Samaru. The samples were transported to the Veterinary Public Health laboratory, ABU, Zaria, for processing. The isolates were further identified as Listeria species by biochemical tests. Antimicrobial susceptibility testing was performed by disc diffusion method. Multiplex PCR was used to identify the virulence genes associated with Listeria monocytogenes. The data were analysed using SPSS V23.0 and Microsoft Excel version 2019 for Windows 10. Odd ratio and 95% CI were determined. Value of P< 0.05 was considered significant.\",\"PeriodicalId\":261891,\"journal\":{\"name\":\"Afro-Egyptian Journal of Infectious and Endemic Diseases\",\"volume\":\"31 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-12-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Afro-Egyptian Journal of Infectious and Endemic Diseases\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21608/aeji.2021.87137.1164\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Afro-Egyptian Journal of Infectious and Endemic Diseases","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21608/aeji.2021.87137.1164","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
患者和方法:在2015年3月至11月期间进行了一项横断面研究，以确定在Zaria销售的牛肉中与单核细胞增生李斯特菌相关的AMR谱和毒力基因。从Zaria市、Tudun-Wada、Danmagaji、Sabon Gari、Kwangila和Samaru的牛肉零售商共收集了240份生牛肉样本。样品被运送到扎里亚ABU兽医公共卫生实验室进行处理。经生化试验进一步鉴定为李斯特菌属。药敏试验采用纸片扩散法。采用多重PCR技术鉴定单核增生李斯特菌相关毒力基因。使用SPSS V23.0和Microsoft Excel version 2019 for Windows 10对数据进行分析。确定奇数比和95% CI。P< 0.05为显著性。
Multiple Anti-microbial Resistance Profile and Molecular Detection of Some Virulence Genes of Listeria Monocytogenes Isolated from Fresh Raw Meat Retailed in Zaria, Northwestern Nigeria
Patients and Methods: A crosssectional study was conducted between March and November 2015 to determine the AMR profile and virulence genes associated with Listeria monocytogenes from beef sold in Zaria. A total of 240 raw beef samples were collected from beef retailers in Zaria city, Tudun-Wada, Danmagaji, Sabon Gari, Kwangila, and Samaru. The samples were transported to the Veterinary Public Health laboratory, ABU, Zaria, for processing. The isolates were further identified as Listeria species by biochemical tests. Antimicrobial susceptibility testing was performed by disc diffusion method. Multiplex PCR was used to identify the virulence genes associated with Listeria monocytogenes. The data were analysed using SPSS V23.0 and Microsoft Excel version 2019 for Windows 10. Odd ratio and 95% CI were determined. Value of P< 0.05 was considered significant.
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