Resistance to the toxicity of conjugative bile salts and Assessment of plasmid borne antibiotic resistance among Indigenous lactobacillus isolates of Pakistan.

Probiotics are the viable microorganisms, which upon digestion exert health-promoting effects on host. Probiotics bacteria must have the potential to serve as a host of antibiotic resistance genes with the risk of transferring the genes in many lactic acid bacteria and other pathogenic bacteria. Many Lactic acid bacteria may carry potentially transmissible plasmid-encoded antibiotic resistance genes. This antibiotic resistance can cause significant harm and suffering to the host. The study aim was to investigate the antibiotic resistance in indigenous lactobacillus isolates and existence of plasmids in the indigenous lactobacillus isolates and relationship between the antibiotic resistance and plasmid. All 79 isolates were offered different resistance pattern to the toxicity of bile salts and were also screened for the antibiotic resistance. The plasmid profiles of all strains were investigated and curing of plasmid was also done. Each strain was tested in triplicate.