Comparative Evaluation of Antimicrobial Efficacy of Calcium Hydroxide, Chitosan, Chlorhexidine and Their Combinations Against Enterococcus Fecalis and Candida Albicans Using Quantitative Real Time Polymerase Chain Reaction – An In Vitro Study

Bacterial invasion of root canal system is crucial for the onset and maintenance of periapical diseases. Hence, one of the goals of the endodontic treatment is to kill micro-organisms in the root canal system. Although, biomechanical preparation and root canal shaping effictively reduces the microbiota, these procedures do not completely eliminate bacteria in the lateral and accessory root canals, isthmi, and apical deltas. Thus, intracanal medication between appointments is recommended to further reduce bacteria in the root canal system.