O. Abakumova, R. P. Baum, N. Y. Ermakova, A. T. Gradyushko, T. N. Guseva-Donskaya, A. Karmenyan, U. M. Koraboyev, V. P. Laptev, V. M. Mechkov, L. Mikhailova, N. G. Panferova, A. G. Rebeko, A. V. Reshetnickov, M. Ryabov, Eugeny P. Stranadko, T. A. Tsvetkova, O. Zhukova
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引用次数: 15
摘要
从螺旋藻蓝藻中获得了一种新的稳定的水溶性光敏剂氯e6，并与n -甲基- d -氨基葡萄糖形成非共价络合物，并对其生物学特性进行了评价，结果如下:以Al-sulfophthalocyanine ' Photosense'(俄罗斯)和寡聚血卟啉- ix混合物' Photogen(俄罗斯)作为允许的参比药物，650 - 900 nm光照射CaOv细胞后，通过DNA合成抑制程度测定其体外光毒性为1 (mu) M (EC50)， 670 nm激光照射PC12细胞后，分别以15和20 J/cm2剂量MTT试验测定其体外光毒性为5 (mu) M (EC65)。
A novel stable water-soluble form of well known photosensitizer chlorin e6 named `Photodithazine' has been obtained from Spirulina Platensis cyanobacteria as a noncovalent complex with N-methyl-D-glucosamine, and its biological characteristics evaluate, which proved to be as follows: in vitro photocytotoxicity was 1 (mu) M (EC50) as determined by the extent of DNA synthesis inhibition in CaOv cells after irradiation with 650 - 900 nm light, and 5 (mu) M (EC65) as determined using MTT test on PC12 cells after irradiation with 670 nm laser light at the doses of 15 and 20 J/cm2, respectively, with Al-sulfophthalocyanine `Photosense' (Russia) and oligomerized hematoporphyrin-IX mixture `Photogen' (Russia) being used as permitted reference drugs.
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