利用“Floratest”光学生物传感器估计放射性核素污染对油菜叶绿素荧光参数诱导的影响

Y. Ruban, V. Illienko, N. Nesterova, O. Pareniuk, K. Shavanova
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引用次数: 3

摘要

采用“Floratest”荧光仪对放射性核素污染土壤上生长的紫薇(Vicia sativa L.)进行了叶绿素荧光(IChH)曲线诱导参数的研究。植物接种了5种可能阻断放射性核素摄取的细菌(放射农杆菌IМВВ-7246、无色固氮杆菌UКМВ-6082、无色芽孢杆菌UКМВ-6003、巨芽孢杆菌UКМВ-5724、豆科根瘤菌bv)。在137Cs(4000±340 Bq/kg)污染的草化土、粗灰化土和泥炭沼泽土壤中生长。研究结果表明,随着无活性叶绿素数量的增加,土壤放射性核素和巨型芽孢杆菌UКМ В-5724的存在对紫薇植物的胁迫最大。此外,在存在巨型芽孢杆菌UKM B-5724的放射性污染影响下,紫薇植物的固定荧光水平(Fst)显著增加,表明光合反应受到抑制。其他细菌在几乎所有类型的土壤中都显示出防辐射特性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Estimation of the effect of radionuclide contamination on Vicia sativa L. induction of chlorophyll fluorescence parameters using "Floratest" optical biosensor
The presented research was aimed to determine the parameters of chlorophyll fluorescence (IChH) curve induction for Vicia sativa L. that were grown on radionuclide contaminated soils by using "Floratest" fluorometer. Plants were inoculated with 5 species of bacteria that might potentially block radionuclide uptake (Agrobacterium radiobacter IМВВ-7246, Azotobacter chroococcum UКМВ-6082, A. chroococcum UКМВ-6003, Bacillus megaterium UКМВ-5724, Rhizobium leguminosarum bv. viceae) and grown in sod-podzolic, сhernozem and peat-bog soils, contaminated with 137Cs (4000±340 Bq/kg). As a result of research, it was determined that the most stressful factors for vetch plants are combination of soil radionuclide and presence of Bacillus megaterium UКМ В-5724, as the number of inactive chlorophyll increased. In addition, the vetch plants significantly increased fixed level of fluorescence (Fst) under the influence of radioactive contamination in presence of Bacillus megaterium UKM B-5724, indicating inhibition of photosynthetic reactions. Other bacteria showed radioprotective properties in almost all types of soil.
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