微流体可变形性分析中的嵌入式单细胞阻抗定位跟踪器

2017 19th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS) Pub Date : 2017-06-18 DOI:10.1109/TRANSDUCERS.2017.7994366

Parham Ghassemi, Xiang Ren, J. Strobl, M. Agah

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引用次数: 0

摘要

本文报道了在一个独特的微流体通道中集成生物阻抗传感和细胞变形性分析，用于单细胞生物物理(生物力学)属性的无标签和无图像分析。全透明的微流控芯片由收缩通道和嵌入铟锡氧化物(ITO)电极阵列组成，通过该阵列可以高分辨率地跟踪细胞位置。因此，在细胞变形过程中，关于细胞传递时间和速度的信息可以很容易地评估，而无需使用视频显微镜。该可变形芯片的独特之处在于，它将传送通道和传感通道分开，从而防止细胞堵塞。该芯片的性能已通过对乳腺癌细胞的单细胞分析得到验证。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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An embedded single-cell impedametric positioning tracker in microfluidic deformability assays

The paper reports the integration of bioimpedance sensing and cell deformability assays in a unique microfluidic channel for label-free and image-free analysis of single-cell biophysical (biomechanical) attributes. The all-transparent microfluidic chip consists of a constriction channel and an array of embedded indium-tin-oxide (ITO) electrodes through which cell position can be tracked with high resolution. Therefore, information regarding cell transit times and velocities during cell deformation can be readily assessed without the use of video microscopy. The deformability chip is also unique in that it separates the delivery and sensing channel thereby preventing cell clogging. The performance of the chip has been verified by single-cell analysis of breast cancer cells.

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2017 19th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS)

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