sPLA2诱导的crocrous duissus水肿涉及PLC和PKC信号,cPLA2的激活和氧化应激

M. Toyama, Caroline R. C. Costa, M. N. Belchor, Danielle P. Novaes, Marcos Antonio de Oliveira, R. Ié, H. H. Gaeta, D. O. Toyama
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引用次数: 5

摘要

从Crotalus durissus terrificus毒液中纯化分离的sPLA2,不含crotapotin (Cdt sPLA2),能够显著增加脂质过氧化,同时增加花生四烯酸(AA)代谢。此外,在Cdt sPLA2注射后15分钟和水肿高峰后(阴性对照),MDA和AA水平升高。因此,氧化应激和ROS在Cdt sPLA2诱导的炎症中起重要作用。另一方面,sPLA2诱导的水肿通过参与磷酸激酶C (PKC)和磷脂酶C (PLC)直接和间接动员花生四烯酸,从而间接刺激细胞质PLA2 (cPLA2)。我们还观察到,与天然产物5-咖啡因-亚油酸(5CQA)和地塞米松(AACOCF3)相比,针对Cdt毒液的特异性抗蛇毒血清对诱导水肿的中和作用不显著。我们的研究结果还表明,由于5CQA是一种有效的抗氧化化合物,与抗蛇毒血清或sPLA2酶活性的抑制相比,天然多酚化合物在抑制水肿方面有改善。因此,我们的研究结果表明花生四烯酸代谢增加与氧化应激之间存在明显的相关性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Edema Induced by sPLA2 from Crotalus durissus terrificus Involves PLC and PKC Signaling, Activation of cPLA2, and Oxidative Stress
sPLA2 from Crotalus durissus terrificus venom, free of crotapotin (Cdt sPLA2), purified and isolated sPLA2, was able to significantly increase lipid peroxidation, which occurred simultaneously with increased arachidonic acid (AA) metabolism. In addition, MDA and AA levels were elevated at 15 min after Cdt sPLA2 injection and after peak edema (negative control). Thus, oxidative stress and ROS play important roles in the inflammation induced by Cdt sPLA2. On the other hand, edema induced by sPLA2 involves the direct and indirect mobilization of arachidonic acid by the involvement of phosphokinase C (PKC) and phospholipase C (PLC), which indirectly stimulates cytosolic PLA2 (cPLA2). We also observed that the specific antivenin against Cdt venom had no significant effect on the neutralization of induced edema compared to the natural products 5-caffeine-linoleic acid (5CQA) and dexamethasone (AACOCF3). Our results also indicate that there was improvement in the inhibition of edema of natural polyphenolic compounds compared to antivenin or inhibition of the enzymatic activity of sPLA2 due to the fact that 5CQA is a potent antioxidant compound. Thus, our results show a clear correlation between increased arachidonic acid metabolism and oxidative stress.
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