磷酸肌酸预处理可减轻离体大鼠心脏缺血再灌注损伤

L. A. Gadjieva, S. Bolevich, V. Jakovlevich, I. A. Omarov, H. A. Ordashev, M. Kartashova
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引用次数: 1

摘要

的目标。研究缺血前立即同时给予磷酸肌酸对离体大鼠心脏逆行灌注时冠脉静脉血流量氧化应激生物标志物及心动力参数的影响。材料和方法。将20只Wistar白化大鼠分为2组:1组(对照组)和2组(实验组),每组10只。采用兰登道夫(Landendorff) Krebs-Henseleit缓冲液对离体大鼠主动脉进行插管和逆行灌注。两组均行缺血-再灌注损伤,全脑缺血20分钟,再灌注30分钟。2组(实验组)缺血前用0.2 mmol/l磷酸肌酸预处理5 min。我们记录了稳定点、再灌注第1分钟和第30分钟时的心脏动力学参数和氧化应激指标。2组再灌注30min磷酸肌酸的影响下,左心室最大、最小压力升高速度分别提高了1.7倍和1.9倍,左心室收缩压和舒张压分别提高了1.5倍和1.6倍。氧化应激生物标志物(脂质过氧化指数、亚硝酸盐、超氧阴离子自由基、过氧化氢)在再灌注1分钟后(分别降低1.2倍、1.4倍、2.8倍、1.9倍)和30分钟后(分别降低1.3倍、2.1倍、1.9倍、2.1倍)也有统计学意义。在缺血发作前5分钟在冠状动脉血流中给予磷酸肌酸对心肌收缩力有保护作用。减少氧化应激和损伤可被认为是磷酸肌酸的保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Creatine phosphate preconditioning reduces ischemiareperfusion injury in isolated rat heart
Aim. To study the effect of simultaneous administration of creatine phosphate immediately before ischemia on cardiodynamic parameters and biomarkers of oxidative stress in the coronary venous blood flow during retrograde perfusion in an isolated rat heart.Materials and methods. 20 Wistar albino rats were divided into 2 groups: group 1 (control) and group 2 (experimental), 10 rats per group. Cannulation and retrograde perfusion of aorta of an isolated rat heart with Krebs–Henseleit buffered solution by Landendorff was performed. Both groups underwent ischemia-reperfusion injury, which included global ischemia for 20 minutes followed by reperfusion for 30 minutes. The group 2 (experimental) was preconditioned with creatine phosphate at a dose of 0.2 mmol/l for 5 min before ischemia. We registered cardiodynamic parameters and indicators of oxidative stress at the point of stabilization, at the 1st and 30th minutes of reperfusion.Results. With the impact of creatine phosphate at the 30th minute of reperfusion in the group 2 in comparison with group 1, there was found an increase in the maximum and minimum speed of pressure elevation in the left ventricle (1.7 and 1.9 times, respectively), and of systolic and diastolic pressure in the left ventricle (1.5 and 1.6 times, respectively). Biomarkers of oxidative stress (lipid peroxidation index, nitrites, superoxide anion radical and hydrogen peroxide) were also statistically significantly lower in the group 2 after the 1st minute of reperfusion (by 1.2 times, by 1.4 times, by 2.8 times and 1.9 times, respectively), and after the 30th minute (1.3 times, 2.1 times, 1.9 times and 2.1 times, respectively).Conclusion. The administration of creatine phosphate into the coronary flow 5 minutes before the onset of ischemia has a protective effect on myocardial contractility. Reduction of oxidative stress and damage can be considered as a protective effect of creatine phosphate.
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