用于从乳腺癌细胞系匀浆中自动提取总RNA的多级，溶剂控制路线

2013 Transducers & Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII) Pub Date : 2013-06-16 DOI:10.1109/TRANSDUCERS.2013.6626763

J. Gaughran, N. Dimov, E. Clancy, T. Barry, T. J. Smith, J. Ducrée

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引用次数: 2

摘要

我们首次报道了在离心微流控平台上通过新颖的、依赖溶剂的路线从细胞匀浆中旋转控制提取总RNA。该系统允许样品制备和综合分析被纳入一个单一的微流体装置。本文还首次报道了一种溶剂触发的阀门机制和纯化总RNA的圆盘萃取性能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Multi-stage, solvent-controlled routing for automated on-disc extraction of total RNA from breast cancer cell line homogenate

We report for the first time rotationally controlled extraction of total RNA from cell homogenate through novel, solvent-dependent routing on a centrifugal micro-fluidic platform. This system allows for sample preparation and integrated analysis to be incorporated on a single microfluidic device. Also reported here for the first time is a solvent-triggered valving mechanism and the performance of on-disc extraction of purified total RNA.

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2013 Transducers & Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII)

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