药用姜和香薷的植物化学筛选及对昆仑山病原菌的抑菌活性研究

Muhammed Hanifa, B. Aminu, Akawu HUSSEINI JOSHUA
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引用次数: 0

摘要

在农业实践中抗生素使用的增加伴随着耐药食源性病原体的发展。据报道,香料和草药是天然抗氧化剂的有效来源。本研究旨在研究生姜和丁香对食物致病菌的抑菌活性。采集新鲜姜、丁香,经鉴定、风干、蒸馏水提取活性成分。对提取液中的黄酮类、单宁类、皂苷类、甾体、苷类、生物碱类和总酚类进行了植物化学分析,并确定了抑制区。从当地制备的Pap (Kunun aya)中分离和鉴定细菌和真菌,随后用于抗菌分析。植物化学筛选表明丁香中含有黄酮类、单宁类、皂苷类、甾体类、苷类、生物碱类和总酚类物质,而姜中不含苷类和单宁类物质。生姜对大肠杆菌的抑菌活性更高,2000µg时对大肠杆菌的抑菌活性最高,为16.73mm; 2000µg时对沙门氏菌的抑菌活性最低,为13.83mm;丁香对金黄色葡萄球菌的抑菌活性最高,为14.8mm,对大肠杆菌的抑菌活性最低,为11.5mm。综上所述,生姜和香薷提取物对食品病原菌具有较好的抗菌作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL ACTIVITY OF Zingiber officinale AND Syzygium aromaticum ON BACTERIA PATHOGENS ISOLATED FROM KUNUN-AYA
The increased use of antibiotics in agricultural practices is accompanied with the development of resistant foodborne pathogens. Spices and herbs have been reported to be potent source of natural antioxidants. This study aimed to assess the antibacterial activity of Zingiber officinale (Ginger) and Syzygium aromaticum (Clove) against bacterial food pathogens. Fresh ginger andclove were collected, identified, air dried and extracted the bioactive compound using distilled water. The extract was used for phytochemical analysis of flavonoids, tannins, saponins, steroids, glycosides, alkaloids and total phenols and determination of inhibition zone. Bacteria and fungi were isolated and identified from locally prepared Pap (Kunun aya) and later used for theantimicrobial analyses. Phytochemical screening shows clove contain flavonoids, tannins, saponins, steroids, glycosides, alkaloids and total phenols whereas glycosides and tannins were absent in ginger. Ginger has more antibacterial activity with the highest zone of inhibition observed in E coli at 16.73mm at 2000µg and the lowest in Salmonella at 13.83mm at 2000µg, clove also shows antibacterial activity with the highest zone of inhibition (14.8mm) in S. aureus at 2000µg and the lowest 11.5mm in E. coli at 2000µg. Thus, from the result obtained, it can be concluded that Zingiber officinale and Syzygium aromaticum extracts possesses effective antimicrobial agents against food pathogens.
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