用于即时诊断的生物发光化学芯片

1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology. Proceedings (Cat. No.00EX451) Pub Date : 2000-10-12 DOI:10.1109/MMB.2000.893856

D. Bartholomeusz, J. Andrade, A. B. Frazier

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引用次数: 4

摘要

讨论了MEMS原型生物芯片器件的设计和进展，该器件使用生物发光作为检测生物流体样品中10-100个总分析物电位的手段。通过在蚀刻方形腔内涂覆铬和银，探索了光增强的方法。通过观察光沿PDMS微通道的透射率，研究了光增强。将含有萤火虫荧光素酶/荧光素溶液的1毫米ATP产生的生物发光光放置在通道和腔室中，通过CCD通过近距离透镜观察光输出。光增强效果由通道或腔室的横截面观察区域的每一升样品体积的CCD计数增加来确定。未包覆硅、包覆铬和包覆银的方形室光输出分别提高了约1-4个CCD计数/nL、3-13个CCD计数/nL和300 - 1,300个CCD计数/nL。未涂覆的PDMS通道增强光约79-101 CCD计数/nL。

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Bioluminescent based chemchip for point-of-care diagnostics

The design and progress of a MEMS prototype biochip device, which uses bioluminescence as a means for detecting a potential of 10-100 total analytes from biofluid samples, is discussed. Light enhancement was explored by coating etched square chambers with chromium and silver. Light enhancement was also investigated by observing the transmittance of light along micro channels molded in PDMS. Bioluminescent light generated from a 1-mM ATP with firefly luciferase/luciferin solution was placed inside the channels and chambers and the light output was observed through a close up lens by a CCD. Light enhancement effectiveness was determined from the CCD count increase per nL of sample volume for the cross sectional viewing areas of the channels or chambers. Uncoated silicon, chromium coated and silver coated square chambers enhanced light output about 1-4 CCD counts/nL, 3-13 CCD counts/nL, and 300 to 1,300 CCD counts/nL respectively. Uncoated PDMS channels enhance light by about 79-101 CCD counts/nL.

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1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology. Proceedings (Cat. No.00EX451)

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