斯里兰卡产鼻甲草(Rhinacanthus nasutus)叶片的抗氧化、杀虫和杀虫活性

V. Bulugahapitiya, Fernando Ptn, Jayasekara Hd
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引用次数: 0

摘要

鼻棘是一种民族药用植物,在传统的医学系统中用于治疗皮肤病。由于在斯里兰卡生长的红毛猴尚无足够的研究,本研究旨在探讨其叶片的抗氧化、抗寄生虫和杀虫活性。采用DPPH自由基清除法和FRAP法,分别以抗坏血酸和FeSO4.7H2O为标准品,评价叶片粗提物的抗氧化能力。采用改良滤纸扩散生物测定法,以0.0010% (w/v)甲醇、乙酸乙酯和叶片水提液为溶剂,测定其对头虱的杀虱效果。采用改良滤纸接触生物测定法对木瓜树和番石榴树上的粉蚧、边缘粉蚧进行了杀虫活性评价。DPPH法的IC50值为514.3 μgmL-1,抗氧化法的FRAP值为594.33±2.93 μmol Fe2+/g。0.0010% (w/v)浓度的甲醇、乙酸乙酯和水提物的中位致死时间(LT50)分别为263、347和676 min。240 min后的LC50(50%死亡率)值为0.0011% (w/v),成虫的LC50值为0.0020% (w/v),分别为31、124和189 h,若虫的LC50值为14、18和29 h。因此,在14 h内,甲醇提取物对粉蚧若虫生活期的控制效果优于成虫,LC50为0.0015% (w/v)。本研究证实了牛蹄草叶片具有良好的杀虫、杀虫活性和显著的抗氧化能力。亚洲医学杂志。[j] .科学通报,2016,36 (4):754-760
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Anti-oxidant, pediculicidal and pesticidal activities of leaves of Rhinacanthus nasutus (Linn) grown in Sri Lanka
Rhinacanthus nasutus is an ethnomedical plant used in the traditional system of medicine for treating skin diseases. As no adequate studies available on R. nasutus grown in Sri Lanka, this study aimed on exploring anti-oxidant, anti-parasitic and pesticidal activity of leaves of the plant. Antioxidant capacity of the crude extract of leaves was evaluated using DPPH free radical scavenging assay and FRAP assay using ascorbic acid and FeSO4.7H2O as the standards respectively. Pediculicidal efficacy was carried out against head lice using 0.0010% (w/v) methanolic, ethyl acetate and aqueous extracts of leaves by following modified filter paper diffusion bioassay. Pesticidal activity of leaves was evaluated using modified filter paper contact bioassay against mealybugs, P. marginatus, resides on papaw and guava trees. The IC50 value of DPPH assay was found to be 514.3 μgmL-1 and FRAP value was found to be 594.33±2.93 μmol Fe2+/g in anti-oxidant assay. The LT50 (median Lethal Time) values for 0.0010% (w/v) concentration of methanolic, ethyl acetate and aqueous extracts were 263, 347 and 676 min respectively in pediculicidal assay. LC50 (50% mortality) value was found to be 0.0011% (w/v) after 240 minutes whereas LT50 values of 0.0020% (w/v) of methanol, ethyl acetate and aqueous fractions of R. nasutus for adult were found as 31, 124 and 189 hours and for nymph were 14, 18 and 29 hours respectively in pesticidal assay. Accordingly, methanolic extract was effective in controlling nymph stage of mealybug life cycle than adults with the LC50 of 0.0015% (w/v) within 14 hours. This study confirmed promising pediculicidal and pesticidal activity and significant anti-oxidant capacity of leaves of R. nasutus. Asian J. Med. Biol. Res. December 2020, 6(4): 754-760
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