W. Putranto, Lilis Suryaningsih, Apon Zaenal, J. Mamangkey
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引用次数: 0
摘要
乳酸菌(LAB)产生的具有与肾素相似特性的蛋白酶称为肾素样蛋白酶(RLP)。本研究旨在了解粪肠球菌1.15产生的RLP的生化特性，为该酶的利用提供依据。研究的第一阶段是观察菌株的生长曲线和生产RLP的潜力，进行生化表征。用Sephadex G-50纯化的粪肠球菌1.15产肾素样蛋白酶(Rennin Like Protease, RLP)分子量为42 kDa，最适温度为40℃，pH为4.5，具有良好的生化特性。MCA/PA值为3.74，与犊牛凝乳酶的MCA值相近，两者比较为1。
Purification and Characterization of Rennin Like Protease from Enterococcus faecium 1.15 Isolated from Indonesia Fermented Bakasam
The protease enzyme produced by Lactic Acid Bacteria (LAB) with characteristics similar to rennin is called Rennin Like Protease (RLP). The purpose of the study was to obtain information on the biochemical characteristics of RLP produced by Enterococcus faecium 1.15 as a basis for the utilization of the enzyme. The first stage of the study was observation of the growth curves and the potential of the isolates to produce RLP, biochemical characterization. Rennin Like Protease (RLP) produced by Enterococcus faecium 1.15 which has been purified using Sephadex G-50 has a molecular weight of 42 kDa with biochemical characters, with an optimum temperature of 40 ℃ and pH of 4.5. The comparison between MCA/PA with a value of 3.74 which is close to the MCA value of Calf Rennet is 1.
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