皮下植入后股骨髓腔内骨髓的再生过程。细胞外基质成分的免疫组织化学和生化分析。

T. Nagasaka, N. Nakashima, Miya Kobayashi, T. Hotta, J. Takeuchi
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引用次数: 0

摘要

将小鼠股骨两端切开后皮下植入同系小鼠腹壁,骨髓组织在骨髓腔内再生。我们每隔一周取出植入骨并评估再生过程。1周后髓腔出现坏死。2周后骨骺出现了基质成分的再生,并随着坏死组织的吸收而进展。3-4周后,形成富含基质成分的粘液瘤基质,导致造血细胞内嵌和增殖。6周后骨髓移植基本完成。免疫组化检查显示3-4周后形成的黏液瘤间质中有大量硫酸软骨素蛋白多糖(PG)和大PG。糖胺聚糖链的生化分析主要显示低硫酸软骨素。粘液瘤间质的形成富含硫酸软骨素PG和大PG,似乎在造血组织的再生中起重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Regenerative process of marrow in the medullary cavities of femurs after subcutaneous implantation. Immunohistochemical and biochemical analysis of the extracellular matrix components.
When mouse femoral bone was subcutaneously implanted after the cutting of both ends into the abdominal wall of syngeneic mouse, bone marrow tissue regenerated in the medullary cavity of the bone. We removed the implanted bones at 1-week intervals and evaluated the regenerative process. The medullary cavity underwent necrosis after 1 week. Regeneration of the stromal components appeared in the epiphyses after 2 weeks and progressed with resorption of the necrotic tissue. After 3-4 weeks, myxomatous stroma rich in matrix components formed resulting in enlodgement and proliferation of hematopoietic cells. Marrow graftment was nearly completed after 6 weeks. Immunohistochemical examination showed a large amount of chondroitin sulfate proteoglycan (PG) and large PG in the myxomatous stroma that formed after 3-4 weeks. Biochemical analysis of the glycosaminoglycan chain primarily revealed low-sulfated chondroitin sulfate. Formation of myxomatous stroma rich in chondroitin sulfate PG and large PG seemes to play an important role in the regeneration of hematopoietic tissue.
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