{"title":"一个简单和廉价的薄层色谱技术比较猪肉和牛肉的清真认证","authors":"Muhammad Ja’far Luthfi","doi":"10.46344/jbino.2021.v10i03.13","DOIUrl":null,"url":null,"abstract":"Nowadays, there are many cases of adulteration of beef with pork. Pork can be found by analyzing the fat. Meat contains fat whose biochemical profile differs between animal species. Based on this principle, we could determine authenticity of given meat by comparing its fat profile. This study aims to develop a thin layer chromatography (TLC) technique to detect pork and determine the chromatographic difference of beef and pork fat. The study revealed that TLC method can be performed to detect pork. TLC was carried out using the hexane-chloroform-methanol (7: 2.5: 1, volume ratio) solvent system. Using this method there were differences in the number of spots and the Rf value between beef and pork fat. Overall, TLC is a method that has the potential to be used as an inexpensive and simple tool for the detection of pork.","PeriodicalId":228982,"journal":{"name":"Journal of Bio Innovation","volume":"9 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A SIMPLE AND INEXPENSIVE THIN LAYER CHROMATOGRAPHY TECHNIQUE TO COMPARE PORK AND BEEF FOR HALAL AUTHENTICATION\",\"authors\":\"Muhammad Ja’far Luthfi\",\"doi\":\"10.46344/jbino.2021.v10i03.13\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Nowadays, there are many cases of adulteration of beef with pork. Pork can be found by analyzing the fat. Meat contains fat whose biochemical profile differs between animal species. Based on this principle, we could determine authenticity of given meat by comparing its fat profile. This study aims to develop a thin layer chromatography (TLC) technique to detect pork and determine the chromatographic difference of beef and pork fat. The study revealed that TLC method can be performed to detect pork. TLC was carried out using the hexane-chloroform-methanol (7: 2.5: 1, volume ratio) solvent system. Using this method there were differences in the number of spots and the Rf value between beef and pork fat. Overall, TLC is a method that has the potential to be used as an inexpensive and simple tool for the detection of pork.\",\"PeriodicalId\":228982,\"journal\":{\"name\":\"Journal of Bio Innovation\",\"volume\":\"9 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-06-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Bio Innovation\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.46344/jbino.2021.v10i03.13\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Bio Innovation","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.46344/jbino.2021.v10i03.13","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A SIMPLE AND INEXPENSIVE THIN LAYER CHROMATOGRAPHY TECHNIQUE TO COMPARE PORK AND BEEF FOR HALAL AUTHENTICATION
Nowadays, there are many cases of adulteration of beef with pork. Pork can be found by analyzing the fat. Meat contains fat whose biochemical profile differs between animal species. Based on this principle, we could determine authenticity of given meat by comparing its fat profile. This study aims to develop a thin layer chromatography (TLC) technique to detect pork and determine the chromatographic difference of beef and pork fat. The study revealed that TLC method can be performed to detect pork. TLC was carried out using the hexane-chloroform-methanol (7: 2.5: 1, volume ratio) solvent system. Using this method there were differences in the number of spots and the Rf value between beef and pork fat. Overall, TLC is a method that has the potential to be used as an inexpensive and simple tool for the detection of pork.
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