A study on the bıoactıvıty of plant extracts obtaıned from Arum maculatum leaves by dıfferent extractıon technıques

Arum maculatum is a highly known plant worldwide for traditional use. The aim of this study is to evaluate the bioactivity of the plant extract obtained using different techniques and solutions. Total fenolic, flavonoid components and antioxidant, antimicrobial and enzyme inhibition activity of the plant extracts (Boiling in water, fermenting in water and USB in methanol) were investigated. Additionally, oil components of the extracts was analysed in GC-MS. As a result of the GC-MS analysis, 18 different fatty acid were determined. Major fatty acid components of extracts were palmitic acid (19.57%), oleic acid (15.25%), linoleic acid (21.84%) and alpha-linolenic acid (15.95%). The plant extracts were also found to be consisting of omega 3-6-9 fatty acid. The results showed that methanolic extracts in USB is produced better and more effective findings than the other extracts. According to the antimicrobial activity experiments, E. coli and S. aureus were the only strains inhibited by the all extracts obtained three different methods. The highest inhibition was recorded against Bacillus subtilis with USB methanol extracts. The only antifungal activity was observed against C. albicans with extracts obtained by boiling in water. Enzyme inhibition activity was very limited with all extracts. Amylase activity was slightly inhibited (14.1%) up to 30 min.