[从冷冻保存的血细胞中分离冷冻保护剂的排除色谱法]。

Folia haematologica (Leipzig, Germany : 1928) Pub Date : 1990-01-01

L Mavrina, S König, A Klein, H Woehlecke, E Richter, G Matthes, R Ehwald

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引用次数: 0

摘要

本文描述了一种用排斥色谱法从冷冻保存的红细胞和血小板浓缩物中分离低分子冷冻保护剂的方法。采用了一种新的囊状包装材料。从细胞中完全分离甘油、二甲基亚砜(DMSO)只需要25 ~ 30分钟。分离后红细胞回收率为86.5%，血小板回收率为75.4%。

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[Exclusion chromatography for the separation of cryoprotective agents from freeze-preserved blood cells].

A method for separating low molecular cryoprotectiva from freeze-conserved erythrocyte- and thrombocyte-concentrates by exclusion chromatography has been described. A new vesicular packing material has been used. Only 25 to 30 minutes are necessary in order to separate glycerol respectively dimethylsulphoxide (DMSO) completely from the cells. 86.5% of the erythrocytes and 75.4% of the thrombocytes were recovered after the separation process.

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Folia haematologica (Leipzig, Germany : 1928)

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