卵母细胞体外成熟后被积云细胞包围的玻璃化与未被积云细胞包围的玻璃化卵母细胞存活率的差异

A. Kasman, B. Santoso, W. Widjiati, A. Hinting, Ni Wajan Tirthaningsih, R. I’tishom, B. Utomo, M. Djati
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引用次数: 0

摘要

本研究旨在评价卵母细胞体外成熟后,被积云细胞包围的卵母细胞玻璃化与未被积云细胞包围的卵母细胞玻璃化的存活率差异。卵母细胞采集采用18g针在卵泡2-6mm处穿刺装有卵母细胞培养基的10cc一次性注射器的抽吸技术。选取细胞质完整的卵母细胞分为两组;卵母细胞被积云细胞包围和未被积云细胞包围。卵母细胞玻璃化用半秸秆转化为液氮10天,继续加热。在5% CO2、38℃、95 ~ 99%的环境条件下,用矿物油覆盖5 ~ 10个卵母细胞,每个卵滴50 μl体外成熟22 h。本研究结果显示,被积云细胞包围的卵母细胞组中正常卵母细胞的形态比例为83.3%,未被积云细胞包围的卵母细胞组中正常卵母细胞的形态比例为70%。经玻璃化加热后体外成熟的卵母细胞,被积云细胞包围的卵母细胞存活率明显高于未被积云细胞包围的卵母细胞。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Viability Differences on Oocyte Vitrification Surrounded by Cumulus Cells and Oocyte Vitrification not Surrounded by Cumulus Cells after In Vitro Maturation
This study aims to evaluate the viability differences on oocyte vitrification surrounded by cumulus cells and oocyte vitrification not surrounded by cumulus cells after in vitro maturation. The oocyte collection was through the aspiration technique of puncturing the 10 cc disposable syringe with 18 G needle filled with oocyte medium on follicle 2-6mm. Oocytes with intact cytoplasm were chosen and divided into two treatment groups; oocytes surrounded by cumulus cells and not surrounded by cumulus cells. The oocyte vitrification used hemi-straw into liquid nitrogen for ten days and continued with warming. In vitro maturation, each was with 50 μl drop for 5-10 oocytes covered with mineral oil in incubator for 22 hours under environmental condition of 5% CO2 , temperature of 38 °C, and humidity of 95-99%. This study resulted in the morphological proportion of normal oocytes was 83.3% in the oocyte group surrounded by cumulus cells and 70% in the oocyte group not surrounded by cumulus cells. The oocyte viability on groups of oocyte surrounded by cumulus cells was insignificantly higher compared to the groups of oocyte not surrounded by cumulus cells after the vitrification and warming followed by in vitro maturation.
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