人结肠肿瘤细胞对生长因子的需求:与细胞分化的关系

Lily Huschtscha , Enrique Rozengurt, Walter F. Bodmer
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引用次数: 10

摘要

研究了表现出不同分化程度的人类结直肠肿瘤系,以确定其生长需求。低分化细胞系SW620、SW480、SW48和SW837在没有外源添加生长因子的Dulbecco改良Eagle培养基中增殖。而中等分化细胞株SW1222、HT29、PC/JW和LS174T仅在添加生长因子的培养基中增殖。SW1222和HT29细胞的生长需要转铁蛋白,而表皮生长因子(SW1222)和亚硒酸钠(HT29)等其他生长促进因子可改善转铁蛋白的生长。PC/JW和LS174T需要胰岛素和转铁蛋白才能达到最佳生长。肿瘤细胞系在添加生长因子的无血清培养基中可以连续传代,在某些情况下比在添加血清的培养基中生长得更好。无血清生长条件将有助于研究结直肠癌细胞系的分化及其与生长因子的相互作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Growth factor requirements of human colorectal tumour cells: Relations to cellular differentiation

Human colorectal tumour lines that exhibit different degrees of differentiation were studied to define their growth requirements. The poorly differentiated cell lines SW620, SW480, SW48 and SW837 proliferated in Dulbecco's modified Eagle's medium without exogenously added growth factors. In contrast, the moderately differentiated cell lines SW1222, HT29, PC/JW and LS174T proliferated only in medium supplemented with growth factor. SW1222 and HT29 cells required transferrin for growth, which was improved by other growth-promoting factors including epidermal growth factor (SW1222) and sodium selenite (HT29). PC/JW and LS174T required both insulin and transferrin for optimal growth. The tumour cell lines could be passaged continuously in serum-free medium supplemented with growth factor and in some cases they grew better than in serum-supplemented medium. The serum-free growth conditions should prove useful for studies on differentiation in colorectal cell lines and their interactions with growth factors.

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