芒果炭疽病相关菌种鉴定及通用LAMP检测方法研究

Plant & Fungal Research Pub Date : 2021-08-31 DOI:10.30546/2664-5297.2021.1.49.

Yukako Hattori, C. Nakashima, Shunsuke Kitabata, Kosuke Naito, A. Hieno, L. V. Alvarez, Keiichiro Motohashi

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引用次数: 1

摘要

摘要:炭疽菌(Colletotrichum gloeosporioides)种复合体含有与多种作物炭疽病相关的植物病原体。在这项研究中，我们设计了一个环介导的等温扩增测定(LAMP)引物，该引物基于从该复合体中分离的物种的分类授权的calmodulingene编码区序列，以快速检测与炭疽病相关的真菌的存在。这种测试可以在种植和销售之间的任何时间点使用。此外，我们还利用从炭疽菌属(Colletotrichum)中分离的菌株检测了该引物的特异性和检测范围。该方法能够特异性地检测出gloeosporioides、C. aoteooa、C.fructicola、C. horii、C. kahawae、C. musae、C. siamense、C. siamense、C. horii等gloeosporioides复合体的成员。可可和热带葡萄。关键词:炭疽病;诊断;系统发育

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Identification of the Colletotrichum species associated with mango diseases and a universal LAMP detection method for C. gloeosporioides species complex

Abstract: The Colletotrichum gloeosporioides species complex contains plant pathogens linked to Anthracnose diseases afflicting various crops. In this study, we designed a loop-mediated isothermal amplification assay (LAMP) primer set based on calmodulin gene coding region sequences from taxonomically authorized isolates of species from this complex to rapidly detect the presence of fungi associated with Anthracnose diseases. This test can be employed at any point between cultivation and sale. Moreover, we examined the specificity and detectable range of this primer set using isolates selected from species of the genus Colletotrichum. This test was able to specifically detect members of the C. gloeosporioides species complex, including C. gloeosporioides, C. aotearoa, C. fructicola, C. horii, C. kahawae, C. musae, C. siamense, C. theobromicola, and C. tropicale. Key Words: Anthracnose, diagnosis, phylogeny, plant disease

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Plant & Fungal Research

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