喜树碱在人白血病细胞K562中分化特性的研究

Paul M.J. McSheehy, Marco Gervasoni, Vito Lampasona, Eugenio Erba, Maurizio D'Incalci
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引用次数: 14

摘要

喜树碱是一种拓扑异构酶I的特异性抑制剂,在细胞处理60分钟后恢复70小时,通过联苯胺染色评估,喜树碱引起人白血病细胞系K562的红系分化。分化通过处理细胞中ϵ-globin和γ-珠蛋白mRNA水平升高和c-myb mRNA下调证实。用非细胞毒性剂量的甲氨蝶呤同步K562细胞,增加喜树碱诱导的分化,但不影响喜树碱诱导的细胞增殖抑制。喜树碱诱导分化和抑制增殖可能有独立的机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Studies of the differentiation properties of camptothecin in the human leukaemic cells K562

Camptothecin, a specific inhibitor of topoisomerase I, caused erythroid differentiation of the human leukaemia cell-line K562, as assessed by benzidine staining at 70 h recovery following a 60 min treatment of the cells. Differentiation was confirmed by increased levels of ϵ-globin and γ-globin mRNA in the treated cells and was accompanied by down-regulation of c-myb mRNA. Synchronisation of K562 cells by non-cytotoxic doses of methotrexate increased the differentiation induced by camptothecin, without affecting the camptothecin-induced inhibition of cellular proliferation. Camptothecin induction of differentiation and inhibition of proliferation may occur by independent mechanisms.

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