三维组织形态调控神经干细胞的分化

2017 19th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS) Pub Date : 2017-07-26 DOI:10.1109/TRANSDUCERS.2017.7994044

Yuki Matsushiro, M. Kato‐Negishi, H. Onoe

{"title":"三维组织形态调控神经干细胞的分化","authors":"Yuki Matsushiro, M. Kato‐Negishi, H. Onoe","doi":"10.1109/TRANSDUCERS.2017.7994044","DOIUrl":null,"url":null,"abstract":"This paper describes the effect of three-dimensional (3D) tissue shape on differentiation ratio of neurons. We cultured mouse neural stem cells (mNSCs) in the closed agarose microchamber sealed with an agarose sheet. We succeeded in fabricating mNSC tissues of various 3D shapes in the closed agarose microchamber and induced differentiation of the lane-shaped mNSC tissues to neurons and glial cells. We confirmed that tissues of thin width and thickness have higher differentiation ratio of neurons than that of spherical-shaped tissue, by changing the width and thickness of the lane shaped tissues.","PeriodicalId":174774,"journal":{"name":"2017 19th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS)","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2017-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Differentiation of neural stem cells regulated by three-dimensional tissue shape\",\"authors\":\"Yuki Matsushiro, M. Kato‐Negishi, H. Onoe\",\"doi\":\"10.1109/TRANSDUCERS.2017.7994044\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This paper describes the effect of three-dimensional (3D) tissue shape on differentiation ratio of neurons. We cultured mouse neural stem cells (mNSCs) in the closed agarose microchamber sealed with an agarose sheet. We succeeded in fabricating mNSC tissues of various 3D shapes in the closed agarose microchamber and induced differentiation of the lane-shaped mNSC tissues to neurons and glial cells. We confirmed that tissues of thin width and thickness have higher differentiation ratio of neurons than that of spherical-shaped tissue, by changing the width and thickness of the lane shaped tissues.\",\"PeriodicalId\":174774,\"journal\":{\"name\":\"2017 19th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS)\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-07-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2017 19th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/TRANSDUCERS.2017.7994044\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2017 19th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/TRANSDUCERS.2017.7994044","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

摘要

本文描述了三维组织形态对神经元分化率的影响。我们在琼脂糖片封闭的琼脂糖微室中培养小鼠神经干细胞(mNSCs)。我们成功地在封闭的琼脂糖微室中制备了各种三维形状的间充质干细胞组织，并诱导其向神经元和神经胶质细胞分化。我们通过改变车道状组织的宽度和厚度，证实了宽度和厚度较薄的组织比球形组织有更高的神经元分化率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Differentiation of neural stem cells regulated by three-dimensional tissue shape

This paper describes the effect of three-dimensional (3D) tissue shape on differentiation ratio of neurons. We cultured mouse neural stem cells (mNSCs) in the closed agarose microchamber sealed with an agarose sheet. We succeeded in fabricating mNSC tissues of various 3D shapes in the closed agarose microchamber and induced differentiation of the lane-shaped mNSC tissues to neurons and glial cells. We confirmed that tissues of thin width and thickness have higher differentiation ratio of neurons than that of spherical-shaped tissue, by changing the width and thickness of the lane shaped tissues.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

2017 19th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS)

自引率

0.00%

发文量