The Coupling of the IoT in the Era of Big Data and the CTC Test Detecting miRNA with Using a Molecular Torch Technique

― While the incidence rate of pancreatic cancer is low, its mortality rate is still higher than expected. These are due to its recurrent tendency and the difficulty of its early diagnosis. Cancer cell floating around in blood (CTC; Circulating Tumor Cell) is one of the causes of cancer recurrence. On the other hand, CTC shows the specific microRNA (miRNA) unlike normal cells. The miR221 and miR155 are representatives of miRNA in pancreatic cancer. Therefore, the detection of miRNA could be an effective tool for predicting metastasis and recurrence of cancer. MiRNA requires quite sensitive detection methods because of their status as very small amount in blood and its complex structure. This study employs a molecular torch technique for miR221 and miR155. Molecular beacon probe applied to miRNA molecules represents the fluorescence signals. The probe can detect miRNA by even the only one per 10 μ l both in animal studies and in vitro experiments. For easier and faster diagnosis of cancer it requires supports from doctors and technical equipments. The study constructed and proposed a specific data sharing system to link the probe with an IoT-application applicable to the real life.