F. Ding, A. A. Krasilnikova, M. Leontieva, L. Stoyanova, A. Netrusov
{"title":"利用高通量测序对地球不同地区的kefi颗粒进行分析","authors":"F. Ding, A. A. Krasilnikova, M. Leontieva, L. Stoyanova, A. Netrusov","doi":"10.55959/msu0137-0952-16-2022-77-4-266-272","DOIUrl":null,"url":null,"abstract":"The taxonomic composition and spatial localization of yeast and bacteria in kefi r grains (KG) obtained for study from diff erent regions of the planet were investigated. The diversity of their microbiome has been demonstrated by high-throughput sequencing of bacterial 16S rRNA genes and the ITS1 region of the 18S-ITS1-5.8S-ITS2-28S complex of yeast rRNA. It has been established that the main representatives of the complex community of KG from diff erent regions are lactic acid bacteria (LAB; lactobacilli, lactococci and Leuconostoc spp. in diff erent ratios), and diff erent types of yeast of the genus Kazachstania (family Saccharomycetaceae). Acetic acid bacteria and a small percentage of yeast Kluyveromyces marxianus were detected in the KG from Tibet, and yeast Pichia kluyveri was detected in the KG from Ossetia.","PeriodicalId":334823,"journal":{"name":"Vestnik Moskovskogo universiteta. Seria 16. Biologia","volume":"55 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Analysis of kefi r grains from diff erent regions of the planet using high-throughput sequencing\",\"authors\":\"F. Ding, A. A. Krasilnikova, M. Leontieva, L. Stoyanova, A. Netrusov\",\"doi\":\"10.55959/msu0137-0952-16-2022-77-4-266-272\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The taxonomic composition and spatial localization of yeast and bacteria in kefi r grains (KG) obtained for study from diff erent regions of the planet were investigated. The diversity of their microbiome has been demonstrated by high-throughput sequencing of bacterial 16S rRNA genes and the ITS1 region of the 18S-ITS1-5.8S-ITS2-28S complex of yeast rRNA. It has been established that the main representatives of the complex community of KG from diff erent regions are lactic acid bacteria (LAB; lactobacilli, lactococci and Leuconostoc spp. in diff erent ratios), and diff erent types of yeast of the genus Kazachstania (family Saccharomycetaceae). Acetic acid bacteria and a small percentage of yeast Kluyveromyces marxianus were detected in the KG from Tibet, and yeast Pichia kluyveri was detected in the KG from Ossetia.\",\"PeriodicalId\":334823,\"journal\":{\"name\":\"Vestnik Moskovskogo universiteta. Seria 16. Biologia\",\"volume\":\"55 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-01-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Vestnik Moskovskogo universiteta. Seria 16. Biologia\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.55959/msu0137-0952-16-2022-77-4-266-272\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Vestnik Moskovskogo universiteta. Seria 16. Biologia","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.55959/msu0137-0952-16-2022-77-4-266-272","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Analysis of kefi r grains from diff erent regions of the planet using high-throughput sequencing
The taxonomic composition and spatial localization of yeast and bacteria in kefi r grains (KG) obtained for study from diff erent regions of the planet were investigated. The diversity of their microbiome has been demonstrated by high-throughput sequencing of bacterial 16S rRNA genes and the ITS1 region of the 18S-ITS1-5.8S-ITS2-28S complex of yeast rRNA. It has been established that the main representatives of the complex community of KG from diff erent regions are lactic acid bacteria (LAB; lactobacilli, lactococci and Leuconostoc spp. in diff erent ratios), and diff erent types of yeast of the genus Kazachstania (family Saccharomycetaceae). Acetic acid bacteria and a small percentage of yeast Kluyveromyces marxianus were detected in the KG from Tibet, and yeast Pichia kluyveri was detected in the KG from Ossetia.
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