Towards Personalized Drug Delivery - Preparation of an Encapsulated Multicompartment System

Single liposomes and vesicles are successfully utilized as delivery vehicles of pharmaceuticals. However limitations of these unilamellar, single compartments led to the development of encapsulated multicompartment systems that establishes the prospect of multicomponent or multifunctional drug delivery systems. So far compartmentalization is restricted to binary systems. To realize a personalized drug delivery, a programmable linkage of n-entities of different content will be needed. Here we present both a programmable DNA-mediated linkage of three distinct vesicle populations and a novel encapsulation protocol. We discuss how the techniques established in this study might be used in personalized healthcare based on customtailored encapsulated multicompartment vesicular drug delivery systems.