MabHLH11转录因子与MaMYB4相互作用,在增加植物东莨菪碱的生物合成方面起着相辅相成的作用

IF 6 1区 农林科学 Q1 AGRONOMY
Zhen Duan, Shengsheng Wang, Zhengshe Zhang, Qi Yan, Caibin Zhang, Pei Zhou, Fan Wu, Jiyu Zhang
{"title":"MabHLH11转录因子与MaMYB4相互作用,在增加植物东莨菪碱的生物合成方面起着相辅相成的作用","authors":"Zhen Duan,&nbsp;Shengsheng Wang,&nbsp;Zhengshe Zhang,&nbsp;Qi Yan,&nbsp;Caibin Zhang,&nbsp;Pei Zhou,&nbsp;Fan Wu,&nbsp;Jiyu Zhang","doi":"10.1016/j.cj.2023.06.011","DOIUrl":null,"url":null,"abstract":"<div><p>The plant natural product scopolin, a coumarin secondary metabolite, has been extensively exploited in flavor, cosmetic, medicine, and other industrial fields. <em>Melilotus albus</em>, a leguminous rotation crop, contains high concentrations of coumarin. The transcriptional regulatory network that controls the flow through the scopolin biosynthesis pipeline in <em>M. albus</em> remains poorly understood. MabHLH11 encodes a basic helix–loop–helix (bHLH) transcription factor whose transcription is positively associated with scopolin accumulation and with the expression of MaMYB4, the bHLH partner of the MYB-bHLH complex. Phylogenetic analysis grouped MabHLH11 in the TRANSPARENT TESTA 8 (TT8) clade of the bHLH IIIf subgroup. The MabHLH11 protein contained an MYB-interacting region and physically interacted with MaMYB4 in yeast and tobacco leaves. Co-overexpression of MabHLH11 with MaMYB4 in <em>M. albus</em> additively increased the expression of <em>UDP-glucosyltransferase</em> (<em>MaUGT79</em>) and induced more scopolin accumulation than occurred under the expression of MabHLH11 alone. MabHLH11 directly targeted the promoter of <em>MaUGT79</em> and the activation of MabHLH11 was strengthened by the presence of MaMYB4. Thus, MaMYB4 enhanced the function of MabHLH11 in upregulating scopolin biosynthesis in <em>M. albus</em>, providing a theoretical basis for scalable production of a high-value plant natural product.</p></div>","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":null,"pages":null},"PeriodicalIF":6.0000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214514123000983/pdfft?md5=40741c2755fc4d192034e5d88b13e14a&pid=1-s2.0-S2214514123000983-main.pdf","citationCount":"1","resultStr":"{\"title\":\"The MabHLH11 transcription factor interacting with MaMYB4 acts additively in increasing plant scopolin biosynthesis\",\"authors\":\"Zhen Duan,&nbsp;Shengsheng Wang,&nbsp;Zhengshe Zhang,&nbsp;Qi Yan,&nbsp;Caibin Zhang,&nbsp;Pei Zhou,&nbsp;Fan Wu,&nbsp;Jiyu Zhang\",\"doi\":\"10.1016/j.cj.2023.06.011\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The plant natural product scopolin, a coumarin secondary metabolite, has been extensively exploited in flavor, cosmetic, medicine, and other industrial fields. <em>Melilotus albus</em>, a leguminous rotation crop, contains high concentrations of coumarin. The transcriptional regulatory network that controls the flow through the scopolin biosynthesis pipeline in <em>M. albus</em> remains poorly understood. MabHLH11 encodes a basic helix–loop–helix (bHLH) transcription factor whose transcription is positively associated with scopolin accumulation and with the expression of MaMYB4, the bHLH partner of the MYB-bHLH complex. Phylogenetic analysis grouped MabHLH11 in the TRANSPARENT TESTA 8 (TT8) clade of the bHLH IIIf subgroup. The MabHLH11 protein contained an MYB-interacting region and physically interacted with MaMYB4 in yeast and tobacco leaves. Co-overexpression of MabHLH11 with MaMYB4 in <em>M. albus</em> additively increased the expression of <em>UDP-glucosyltransferase</em> (<em>MaUGT79</em>) and induced more scopolin accumulation than occurred under the expression of MabHLH11 alone. MabHLH11 directly targeted the promoter of <em>MaUGT79</em> and the activation of MabHLH11 was strengthened by the presence of MaMYB4. Thus, MaMYB4 enhanced the function of MabHLH11 in upregulating scopolin biosynthesis in <em>M. albus</em>, providing a theoretical basis for scalable production of a high-value plant natural product.</p></div>\",\"PeriodicalId\":10790,\"journal\":{\"name\":\"Crop Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":6.0000,\"publicationDate\":\"2023-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2214514123000983/pdfft?md5=40741c2755fc4d192034e5d88b13e14a&pid=1-s2.0-S2214514123000983-main.pdf\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Crop Journal\",\"FirstCategoryId\":\"91\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2214514123000983\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Crop Journal","FirstCategoryId":"91","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214514123000983","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 1

摘要

植物天然产物东莨菪碱是一种香豆素次生代谢物,已在香料、化妆品、医药和其他工业领域得到广泛开发。豆科轮作作物 Melilotus albus 含有高浓度的香豆素。人们对控制白千层中香豆素生物合成管道流量的转录调控网络仍然知之甚少。MabHLH11 编码一种基本螺旋-环-螺旋(bHLH)转录因子,其转录与东莨菪碱的积累以及 MaMYB4(MYB-bHLH 复合物的 bHLH 伙伴)的表达呈正相关。系统发育分析将 MabHLH11 归入了 bHLH IIIf 亚群的 TRANSPARENT TESTA 8(TT8)支系。MabHLH11蛋白含有一个MYB相互作用区,并在酵母和烟草叶片中与MaMYB4发生物理相互作用。与单独表达MabHLH11相比,MabHLH11与MaMYB4在M. albus中的共重表达会增加UDP-葡萄糖基转移酶(MaUGT79)的表达,并诱导更多的东莨菪碱积累。MabHLH11直接靶向MaUGT79的启动子,MaMYB4的存在加强了MabHLH11的激活。因此,MaMYB4增强了MabHLH11在上调白僵菌东莨菪碱生物合成中的功能,为可规模化生产高价值植物天然产品提供了理论基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The MabHLH11 transcription factor interacting with MaMYB4 acts additively in increasing plant scopolin biosynthesis

The plant natural product scopolin, a coumarin secondary metabolite, has been extensively exploited in flavor, cosmetic, medicine, and other industrial fields. Melilotus albus, a leguminous rotation crop, contains high concentrations of coumarin. The transcriptional regulatory network that controls the flow through the scopolin biosynthesis pipeline in M. albus remains poorly understood. MabHLH11 encodes a basic helix–loop–helix (bHLH) transcription factor whose transcription is positively associated with scopolin accumulation and with the expression of MaMYB4, the bHLH partner of the MYB-bHLH complex. Phylogenetic analysis grouped MabHLH11 in the TRANSPARENT TESTA 8 (TT8) clade of the bHLH IIIf subgroup. The MabHLH11 protein contained an MYB-interacting region and physically interacted with MaMYB4 in yeast and tobacco leaves. Co-overexpression of MabHLH11 with MaMYB4 in M. albus additively increased the expression of UDP-glucosyltransferase (MaUGT79) and induced more scopolin accumulation than occurred under the expression of MabHLH11 alone. MabHLH11 directly targeted the promoter of MaUGT79 and the activation of MabHLH11 was strengthened by the presence of MaMYB4. Thus, MaMYB4 enhanced the function of MabHLH11 in upregulating scopolin biosynthesis in M. albus, providing a theoretical basis for scalable production of a high-value plant natural product.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Crop Journal
Crop Journal Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
9.90
自引率
3.00%
发文量
638
审稿时长
41 days
期刊介绍: The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics. The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信