土壤绿色微藻Edaphochloris gen. 11 .一新属(小球藻科,微藻科)

A. Temraleeva, E. Krivina, O. Boldina
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引用次数: 0

摘要

小型单细胞绿球藻(绿藻)的细胞形态简单统一,使其难以正确识别,因此对其物种多样性的研究仍然具有重要意义。现在人们普遍认为,创造一个新的、更自然的“小绿球”分类的最佳方法是结合形态学、生态学、生理学和分子系统发育方法。菌株ACSSI 104和ACSSI 144分别从俄罗斯伏尔加格勒地区的solonetz和栗子土中分离得到。它们的细胞具有典型的纳米氯化物样形态:单个球形或亚球形细胞,叶绿体单一,顶叶,杯状,不含类pypyrenox,但含有淀粉粒。老细胞在细胞质中积聚大量油滴。由2−4个常孢子繁殖。两种菌株的常孢子大小相等,并通过孢子囊细胞壁的破裂而释放。未观察到游动孢子和有性繁殖。这两个菌株都是与Marvania属、Pumiliosphaera属、Nannochloris属和Gloeotila属密切相关的独立集群的一部分。通过对其形态特征(细胞大小、叶绿体类型)、超微结构特征(细胞壁和片层系统结构)、繁殖特征(仅靠自孢子无性繁殖)和生境(土壤)的比较分析,以及对其18S rRNA基因和ITS2二级结构的分析,我们与模式种E. andreyevae提出了一个新属Edaphochloris。该报告是根据2021年国际参与的第五届全俄科学会议“藻类:系统分类学、生态学和监测使用问题”的材料编写的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A new genus of soil green microalgae Edaphochloris gen. nov. (Chlorellaceae, Trebouxiophyceae)
The problem of studying the species diversity of small single-celled green coccoid microalgae (Chlorophyta) is still relevant, since their simple and uniform cellular morphology makes it extremely difficult to correctly identify them. It is now generally accepted that the best approach for creating a new, more natural classification of “little green balls” is an approach that combines morphological, ecological, physiological and molecular phylogenetic methods. Strains ACSSI 104 and ACSSI 144 were isolated from solonetz and chestnut soil (Volgograd region, Russia). Their cells have a typical Nannochloris-like morphology: single spherical or subspherical cells, chloroplast single, parietal, cup-shaped, without pyrenoid, but with starch grains in it. Old cells accumulate numerous oil droplets in cytoplasm. Reproduction by 2−4 autospores. Autospores of both strains are equal size and exhibit liberation by rupture of sporangium cell wall. Zoospores and sexual reproduction not observed. Both strains are part of independent cluster closely related to genera Marvania, Pumiliosphaera, Nannochloris and Gloeotila. A comparative analysis of morphological (cell size, chloroplast type) and ultrastructural characteristics (structure of cell wall and lamellar system), features of reproduction (asexual reproduction by autospores only) and habitat (soil) with other members of the Nannochloris-clade, as well as results of analysis of 18S rRNA gene and ITS2 secondary structure of the studied strains, allowed us to propose a new genus – Edaphochloris with the type species – E. andreyevae. The report was prepared based on the materials of the V All-Russian Scientific Conference with international participation "Algae: problems of systematics, ecology and use in monitoring", 2021.
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