印度尼西亚茂物植物园兰花感染齿舌环斑病毒(ORSV)的植物-病毒相互作用

Proceedings of the 1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia Pub Date : 1900-01-01 DOI:10.4108/EAI.2-5-2019.2284701

Mahfut Mahfut, B. Daryono, A. Indrianto, Soesamto Somowiyarjo

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引用次数: 2

摘要

由牙釉质环斑病毒(ORSV)感染引起的生物胁迫导致光合作用速率下降。本研究旨在通过分析rbcL和外壳蛋白基因来确定植物与病毒相互作用的模式。病毒源采集自茂物植物园。利用逆转录聚合酶链式反应(RT-PCR)和聚合酶链式反应(PCR)分别检测叶绿体和ORSV。使用DNASTAR和MEGA Beta程序分析核苷酸和氨基酸序列的变化。叶绿体DNA和病毒RNA扩增分别得到±599 bp和±474 bp的扩增子。序列核苷酸分析显示位点突变增加了Ser、Glu、Pro、dan Arg氨基酸的合成。这项研究表明，核苷酸和氨基酸序列的变化被认为是宿主病毒对印度尼西亚自然环境的适应和生理反应。

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Plant-Virus Interaction on Orchids Infected Odontoglossum ringspot virus (ORSV) in Bogor Botanical Garden, Indonesia

Biotic stress by Odontoglossum ringspot virus (ORSV) infection causes a decrease in the rate of photosynthesis. This study aimed to determine the pattern of plant-virus interactions through analysis of rbcL and coat protein genes. Virus source was collected from Bogor Botanical Garden. Detection of chloroplast and ORSV using Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and Polymerase Chain Reaction (PCR), respectively. Analyzed change of nucleotide and amino acid sequences using DNASTAR and MEGA Beta programs. Chloroplast DNA and virus RNA amplification managed to get amplicons at ±599 bp and ±474 bp, respectively. Sequence nucleotides analysis showed point mutations with increased synthesis of Ser, Glu, Pro, dan Arg amino acids. This study revealed a change in sequence of nucleotides and amino acids were thought to be adaptations and physiological responses of host-virus to natural environments in Indonesia.

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Proceedings of the 1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia

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