Simmental Identification of SNP BTA10 to Twinning Birth Trait of Simmental - Ongole Grade Crossing

Increasing of beef cattle population can be done by utilizing the genetic potential of twinning birth, which can be identified through exploration of quantitative trait loci markers (QTL) at locus chromosome 10 (BTA10). The aim of this research was to identify single nucleotide polymorphisms (SNPs) of BTA10 molecular markers on the trait of twinning birth. Ongole Grade (OG) cattle 22 heads and SIMPO (Simental – OG crossing) cattle 86 heads, they were cows and its twin or single calves, taken its blood as samples research. The sequencing method was used to detect SNPs on BTA10 and SNAP to demonstrate validation markers of the twinning birth traits. The results showed that SNP verification in BTA 10 was not related to genetic of the twinning birth trait in OG or SIMPO cattle, but SNP A/T position of 75 bp at consensus area in its clamp sequence of locus Hapmap22923-BTA-129564 showed significant association with the twinning birth trait only in SIMPO cattle. It was concluded, SNP in BTA 10 had not been proven to be related to genetic traits of twinning birth in OC cattle, but SNP A/T in 75 bp position was significantly associated with SIMPO cattle.