P40 Brain Slices Plastination Using Methanol for Dehydration

Human brain slices, 4mm thick, were plastinated using methanol (methyl alcohol) as the dehydration medium instead of acetone. The slices were processed using the standard P40 technique and it was established that the use of methanol as a dehydration medium would not generate problems in plastination of brain slices. Some particularities are to be regarded only in the impregnation phase of the plastination process.