A. Mladenović, M. Perović, J. Milanović, D. Kanazir, L. Rakić, D. S. Ruzdijic
{"title":"帕金森大鼠纹状体的羟多巴胺损伤导致多巴胺受体mRNA的改变","authors":"A. Mladenović, M. Perović, J. Milanović, D. Kanazir, L. Rakić, D. S. Ruzdijic","doi":"10.2298/JMH0203275M","DOIUrl":null,"url":null,"abstract":"The effects of four-site intrastriatal 6-hydroxydopamine (6-OHDA) lesions were examined in adult male rats. Five days after the lesions the animals were checked for specific rotational behavior induced by middle dose of amphetamine and the results confirmed the effectiveness of the lesions. The RNAs from the striatum were isolated at different time points after the lesion, and the RT-PCR analyse were performed for the D1 and D2 receptor mRNA. The results show a decline in the D2 receptor mRNA level (40%) at 6 h and 24 h points while this change was not observed seven days after the lesion. In contrast, no statistically significant changes in the level of the D1 receptor mRNA after the lesion at any time point were found.","PeriodicalId":287983,"journal":{"name":"Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry","volume":"140 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"6-HYDROXYDOPAMINE LESIONS OF THE STRIATUM LEAD TO THE ALTERATIONS OF DOPAMINE RECEPTOR mRNA IN PARKINSONIAN RATS\",\"authors\":\"A. Mladenović, M. Perović, J. Milanović, D. Kanazir, L. Rakić, D. S. Ruzdijic\",\"doi\":\"10.2298/JMH0203275M\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The effects of four-site intrastriatal 6-hydroxydopamine (6-OHDA) lesions were examined in adult male rats. Five days after the lesions the animals were checked for specific rotational behavior induced by middle dose of amphetamine and the results confirmed the effectiveness of the lesions. The RNAs from the striatum were isolated at different time points after the lesion, and the RT-PCR analyse were performed for the D1 and D2 receptor mRNA. The results show a decline in the D2 receptor mRNA level (40%) at 6 h and 24 h points while this change was not observed seven days after the lesion. In contrast, no statistically significant changes in the level of the D1 receptor mRNA after the lesion at any time point were found.\",\"PeriodicalId\":287983,\"journal\":{\"name\":\"Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry\",\"volume\":\"140 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1900-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2298/JMH0203275M\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2298/JMH0203275M","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
6-HYDROXYDOPAMINE LESIONS OF THE STRIATUM LEAD TO THE ALTERATIONS OF DOPAMINE RECEPTOR mRNA IN PARKINSONIAN RATS
The effects of four-site intrastriatal 6-hydroxydopamine (6-OHDA) lesions were examined in adult male rats. Five days after the lesions the animals were checked for specific rotational behavior induced by middle dose of amphetamine and the results confirmed the effectiveness of the lesions. The RNAs from the striatum were isolated at different time points after the lesion, and the RT-PCR analyse were performed for the D1 and D2 receptor mRNA. The results show a decline in the D2 receptor mRNA level (40%) at 6 h and 24 h points while this change was not observed seven days after the lesion. In contrast, no statistically significant changes in the level of the D1 receptor mRNA after the lesion at any time point were found.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
