微生物诱导方解石降水对堆肥技术生物修复原油污染土壤压实特性的影响

Musa K. Alhassan, K. Umar
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摘要

夯实作为一种改善土壤工程性能的土壤改良方法,在修复污染土壤上开展了微生物诱导方解石降水(MICP)对夯实特性的影响研究。从储存原油的卡杜纳炼油厂W区石油污染土壤中分离到地衣芽孢杆菌。用鸡粪中提取的有机液体营养液(CD)按土壤样品干重的10%、20%、30%和40%的阶梯式浓度对细菌进行强化,并用于污染土壤的水化处理。在4个培养皿中保存3个月。初始总烃含量为98g/kg。采用MICP技术对生物修复土壤进行菌液处理。结果表明,30% CD处理的样品除油率最高,达到89.8%。添加地衣芽孢杆菌溶液后,最大干密度增加,1.5×108细胞/ml处理的样品最大干密度为1.93Mg/cm3。当浓度分别为1.5×108和2.4 ×109 cells /ml时,地衣芽孢杆菌悬浮液的最适含水量(OMC)随浓度的增加而增加,分别为14.4% ~ 15.8%。采用MICP技术,在1.5×108细胞/ml细菌溶液条件下,30% CD处理后的土壤压实特性明显改善,可作为垃圾处理的衬垫材料。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Influence of Microbial-Induced Calcite Precipitation on Compaction Characteristics of Bioremediated Crude Oil Contaminated Soil using Composting Technique
Compaction as a soil improvement method of improving the engineering performance of soil was carried out on remediated contaminated soil with the aim of assessing the influence of microbial induced calcite precipitation (MICP) on compaction characteristics. Autochthonous bacteria was identified as Bacillus licheniformis and isolated from oil contaminated soil, collected from Kaduna refinery at Area W where the crude oil was stored. The bacteria were enhanced with organic liquid nutrient extracted from chicken droppings (CD) in stepped concentration of 10, 20, 30, and 40% by dried weight of soil sampleand used to hydrate the contaminated soil. The mixtures were kept in four microcosms for 3 months. The initial total hydrocarbon content was 98g/kg. The bioremediated soil was treated with bacteria solution through MICP technique. The results revealed that sample treated with 30% CD yielded the highest removal efficiency of 89.8% of oil removal. Maximum Dry Density increased with addition of B. licheniformis solution to a peak value of 1.93Mg/cm3 for sample treated with 1.5×108 cell/ml. Optimum moisture content (OMC) increased with increased in B. licheniformis suspension from 14.4 to 15.8% when treated with 1.5×108 and 2.4 ×109 cell/ml respectively. Compaction characteristics of soil treated with 30% CD were greatly improved at 1.5×108 cell/ml bacteria solution using MICP technology and could be used as liner material in waste management.
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