E. Skurikhin, M. Zhukova, N. Ermakova, L. Kogai, A. Dygai
{"title":"体外研究可能有助于供体选择和评估CD8+ t细胞重编程的有效性:一项试点研究的经验","authors":"E. Skurikhin, M. Zhukova, N. Ermakova, L. Kogai, A. Dygai","doi":"10.3390/ecb2023-14289","DOIUrl":null,"url":null,"abstract":"The survival and cytotoxicity of reprogrammed human CD8+ T-cells (hrT-cells) were evaluated in a culture of cancer stem cells (CSCs) isolated from a patient with small-cell lung cancer (SCLC). T-cells were isolated from the blood of healthy volunteers and patients with lung diseases. Reprogramming with MEK and PD-1 inhibitors increased the survival and cytotoxicity of allogeneic T-cells in vitro. The positive effect of reprogramming is more pronounced in patients with lung diseases than in healthy donors. Autologous hrT-cells showed high effectiveness in eliminating CSCs. Thus, in vitro studies are significant in the selection of potential cell donors and evaluating the effectiveness of their reprogramming.","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"In Vitro Studies May Be Useful in Donor Selection and Evaluating the Effectiveness of CD8+ T-Cell Reprogramming: Experience of a Pilot Study\",\"authors\":\"E. Skurikhin, M. Zhukova, N. Ermakova, L. Kogai, A. Dygai\",\"doi\":\"10.3390/ecb2023-14289\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The survival and cytotoxicity of reprogrammed human CD8+ T-cells (hrT-cells) were evaluated in a culture of cancer stem cells (CSCs) isolated from a patient with small-cell lung cancer (SCLC). T-cells were isolated from the blood of healthy volunteers and patients with lung diseases. Reprogramming with MEK and PD-1 inhibitors increased the survival and cytotoxicity of allogeneic T-cells in vitro. The positive effect of reprogramming is more pronounced in patients with lung diseases than in healthy donors. Autologous hrT-cells showed high effectiveness in eliminating CSCs. Thus, in vitro studies are significant in the selection of potential cell donors and evaluating the effectiveness of their reprogramming.\",\"PeriodicalId\":265361,\"journal\":{\"name\":\"The 2nd International Electronic Conference on Biomedicines\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-03-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The 2nd International Electronic Conference on Biomedicines\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3390/ecb2023-14289\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The 2nd International Electronic Conference on Biomedicines","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/ecb2023-14289","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
In Vitro Studies May Be Useful in Donor Selection and Evaluating the Effectiveness of CD8+ T-Cell Reprogramming: Experience of a Pilot Study
The survival and cytotoxicity of reprogrammed human CD8+ T-cells (hrT-cells) were evaluated in a culture of cancer stem cells (CSCs) isolated from a patient with small-cell lung cancer (SCLC). T-cells were isolated from the blood of healthy volunteers and patients with lung diseases. Reprogramming with MEK and PD-1 inhibitors increased the survival and cytotoxicity of allogeneic T-cells in vitro. The positive effect of reprogramming is more pronounced in patients with lung diseases than in healthy donors. Autologous hrT-cells showed high effectiveness in eliminating CSCs. Thus, in vitro studies are significant in the selection of potential cell donors and evaluating the effectiveness of their reprogramming.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
