The Effects of Temperature and Salinity on the Hatching of Fertilized Eggs of Diodon holocanthus

The effects of temperature and salinity on the hatching of the fertilized eggs of Diodon holocanthus were studied. The temperature (A) was set at 21 ̊C, 24 ̊C, 27 ̊C, 30 ̊C and 33 ̊C. The salinity (B) was set as 5 gradients of 18, 22, 26, 30 and 34, and three repetitions were set for each group. Each has parallel 100 fertilized eggs. The results showed that the incubation time decreased gradually with the increase of temperature. When the temperature was between 24 ̊C and 33 ̊C, the incubation time gradually decreased with the increase of salinity, and the incubation time at 18 ̊C was significantly higher than that of other experimental groups. When the salinity was 26 34, the hatching rate gradually decreased with the increase of temperature, and the hatching rate at 24 ̊C was significantly higher than that in other experimental groups (P < 0.05). When the temperature was 24 ̊C 27 ̊C, the incubation rate was significantly higher than that of other experimental groups when the salinity was 30 ̊C. When the temperature is 30 ̊C 33 ̊C, the hatching rate decreases with the increase of salinity. The survival rate first increased and then decreased with the increase of temperature. When the temperature is 24 ̊C 27 ̊C, the survival rate first increases and then decreases with the increase of salinity, and the survival rate of the 30 groups is higher than that of other groups. At the temperature of 30 ̊C, there was no significant difference between the experimental groups, but at the temperature of 33 ̊C, the survival rate decreased significantly with the increase of salinity. In terms of single factor, temperature and salinity have extremely significant effects on hatching time, hatching rate and survival rate of the fertilized eggs of Diodon holocanthus (P < 0.01), and the interaction between salinity and temperature has extremely significant effects on hatching and survival rate of the fertilized eggs of Diodon holocanthus (P < 0.01). In conclusion, the optimal incubation temperature for the fertilized eggs of the Diodon holocanthus is about 27 ̊C, and the optimal incubation salinity is between 26 ̊C and 30 ̊C.