流动溶酶法及其生物医学应用:大群体中单细胞的细胞质分析

2009 IEEE 22nd International Conference on Micro Electro Mechanical Systems Pub Date : 2009-03-27 DOI:10.1109/MEMSYS.2009.4805320

W.C. Lee, F. Kuypers, Y. Cho, A. Pisano

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引用次数: 0

摘要

我们提出了一种新的单细胞分析仪，流溶法，以测量大细胞群中的细胞质成分。目前的流动溶解法在一个连续的微流体通道中执行并同步三个功能(细胞检测、细胞裂解和成分传感)，从而实现宽目标成分的高通量测量(20个细胞/分钟)。在实验研究中，我们测量了每个红细胞的胞质成分(Ca++离子)，并验证了混合细胞群体中单个细胞的群体分析。因此，这项工作表明，目前的流动滴定法是有用的，以表征复杂的细胞群，这是各种生物医学研究所需要的。

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Flow-Lysometry and its Biomedical Application: Cytosolic Analysis of Single Cells in Large Populations

We present a novel single-cell analyzer, flow-lysometry, to measure cytosolic components in large cell populations. The present flow-lysometry performs and synchronizes three functions (cell-detection, cell-lysis, and component-sensing) in a continuous microfluidic channel, thus achieving high-throughput measurements (20 cells/min) of wide target components. In the experimental study, we measure the cytosolic component (Ca++ ion) from each single RBC and verify population analysis of single cells in mixed cell populations. Thus, this work shows that the present flow-lysometry is useful to characterize complex cell populations, which is required for various biomedical studies.

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2009 IEEE 22nd International Conference on Micro Electro Mechanical Systems

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